Determination of the minimum lethal concentration of glufosinate of ammonia in different plant materials of banana cv. ‘Grande naine’ (Musa AAA)
Journal Title: Biotecnología Vegetal - Year 2010, Vol 10, Issue 3
Abstract
Genetic breeding of bananas by genetic transformation requires an efficient selection system. One of the selection markers most widely used is the bar gene from Streptomyces hygroscopicus, which encodes phosphinothricin acetyltransferase and confers resistance to phosphinothricin and glufosinate-ammonium, the active ingredients in several commercial herbicides such as BASTA ® , Finale ® and Liberty ® . The present investigation was aimed to determine the minimum lethal concentration of glufosinate-ammonium (Finale ®) on embryogenic cell aggregates, in vitro cultured shoots and plants grown in greenhouse of banana cv. ‘Grande naine’ (Musa AAA). Different concentrations of this herbicide were used in the culture embryogenic cell clusters (5.0, 10.0, 15.0, 20.0, 25.0 mg l -1 ), in vitro cultured shoots (0, 1.0, 1.5, 2.0, 3.0, 4.0 mg l -1 ). This was also applied to the foliage of plants in greenhouse (0, 5.0, 10.0, 20.0, 30.0, 40.0 g l -1 ). Results showed that the minimum lethal concentration in embryogenic cell clusters was 20.0 mg l -1 glufosinate ammonium,in in vitro shoots 3.0 mg l -1 and in plants in greenhouse 30.0 g l -1 . Results also demonstrated that the use of glufosinate ammonium as a selective agent of transformants carrying the bar gene is possible.
Authors and Affiliations
Maritza Reyes, Rafael G. Kosky, Idalmis Bermúdez-Caraballoso, Borys Chong-Pérez
Keywords
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