DETERMINATION OF VAGINAL GEL COMPOSITION ON THE BASIS OF BIOPHARMACEUTICAL AND RHEOLOGICAL RESEARCHES
Journal Title: Annals of Mechnikov Institute - Year 2018, Vol 0, Issue 4
Abstract
Aim. The aim of the work is to determine the content of gelling agent in the composition of the vaginal gel for the treatment of urogenital symptoms during the period of women's hormonal instability and to determine the concentration of a non-aqueous solvent propylene glycol in the gel to provide release of the active ingredient, resveratrol. Materials and methods. As objects of the study samples of gels with resveratrol and hyaluronic acid with varying concentrations of gelling agent aristoflex AVC and non-aqueous solvent propylene glycol have been taken. Biopharmaceutical studies by equilibrium dialysis through a semipermeable membrane for 6 hours were performed to select propylene glycol concentration. The optical density of the samples obtained was determined using Evolution 60-S spectrophotometer. The concentration of the gelling agent was determined by rheological performance using the Rheolab QC rheometer, by Anton Paar, Austria. Results. In the course of the study, a comparison was made between resveratrol release from samples with propylene glycol concentration of 10% and 15%. At 6th hour of the experiment, a larger amount of resveratrol, which has passed to the solution, was observed in a sample with propylene glycol concentration 15%. It has been established that an increase in the concentration of propylene glycol contributes to an increase in the rate of the active ingredient release from gel samples. Thus, for further studies on the development of vaginal gel composition, the concentration of non-aqueous solvent was chosen to be 15%. At the next stage, the choice of gel-former concentration in the gel was made. In the course of the study, rheological properties of samples with gel-former concentration of 1%, 1.5% and 2% have been compared. The obtained rheograms of gel samples indicate that all systems are coagulating with a pseudoplastic flow type and a certain degree of thixotropy. A sample with the concentration of 1% is a liquefied system that has practically no structure, as evidenced by the large difference between initial and final viscosity. An increase in the concentration of gelling agent to 2% leads to a strengthening of the structure. The sample with Aristoflex concentration 1,5% has optimal rheological parameters that can provide high biological availability of active substances. Conclusions. It has been established that the optimal concentration of PG, which contributes to maximal release of the active substance, is 15% of the total mass of the sample. According to the results of rheological research, the rational content of the gel-former in the gel is 1,5%.
Authors and Affiliations
O. I. , Ivaniuk, T. G. , Yarnykh, V. O. , Hrudko, І. V. Kovalevska
Keywords
Related Articles
- EP ID EP612813
- DOI 10.5281/zenodo.2547460
- Views 172
- Downloads 0
COMPARATIVE ASESSMENT OF BACTERIAL EXCRECTION DEGREE DEPENDING FROM SENSIVITY PROFILE OF MYCOBACTERIUM TUBERCULOSIS TO ANTITUBERCULOSIS DRUGS
One of the important part of the epidemiological component of tuberculosis pathomorphosis in modern conditions is the change in important biological properties of the pathogen, not only in the form of increased frequency...
COMPARATIVE ANALYSIS OF FREON EXTRACTS FROM LIME FLOWERS CROPPED IN 2016 AND 2017 YEARS
Introduction. The article is devoted to gas-liquid-chromatography analysis of the freon extracts taken from lime flowers cropped in 2016 and 2017 years. It’s well-known that quantitative content of a sum of extractives o...
POLYMORPHISM PREVALENCE OF TLR 9 GENE IN PATIENTS WITH INFECTIOUS MONONUCLEOSIS CAUSED BY EPSTEIN-BARR VIRUS
Annotation. The prevalence of polymorphism -1486 T/C of TLR-9 gene in 52 patients with infectious mononucleosis (IM) caused by the Epstein-Barr virus was studied. Based on the results obtained, three main genotypes -1486...
PROSPECTS FOR USE OF CONDENSED GASES AND SUPERCRITICAL FLUIDS IN PHYTOCHEMICAL PRODUCTION
In the given review article analysis of the literature and patent sources concerning main methods for intensification of ex-traction processes of medicinal vegetative raw materials – use of condensed gases and supercriti...
AGE-RELATED CHANGES OF SENSIBILIZATION PROFILES TO INDOOR INHALATION ALLERGENS IN THE PATIENTS OF THE SOUTHERN REGION OF UKRAINE
Introduction: It is known that epidemiology of allergic diseases is affected by range of plants, animal and insect allergens, social and living conditions, nature of food habits of certain populations, sex and age of pat...