DEVELOPMENT AND APPLICATION OF Pasteurella multocida SEROTYPE A:1 WHOLE CELL BASED COAGGLUTINATION TEST (CAT) FOR THE RAPID DETECTION OF FOWL CHOLERA CAUSING ORGANISMS
Journal Title: Journal of Experimental Biology and Agricultural Sciences - Year 2017, Vol 5, Issue 0
Abstract
Pasteurella multocida whole cell antigen based coagglutination test was optimized in the laboratory for the specific and sensitive identification of bacteria causing fowl cholera in a simple and cost effective manner. The test showed 100% specificity and sensitivity up to 3.4 ×106 formalinized bacterial cells. For testing stability, sensitized coagglutination test reagents were stored at 40C for 1-4 months was subjected to reactions with inactivated P. multocida type A:1, A:3 and A:4 and non Pasteurella cultures at the end of each month. The optimized test also detected P. multocida type A:1, A:4 in experimentally infected birds (n=10) indicating the potential application of the reagent to detect all possible somatic types of A capsular type which are etiological agents of Fowl cholera. P.multocida type B:2 experimentally infected mice (n=4) tissues also showed coagglutination with the reagent exemplify its wider applicability in field diagnosis.
Authors and Affiliations
Stanzin Zadon, Vinod Kumar Chaturvedi, Praveen Kumar Gupta, Varsha Paladan, Perumal Nanda Kumar
Keywords
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- EP ID EP427745
- DOI 10.18006/2017.5(6).767.773
- Views 53
- Downloads 0
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