Abstract

Abstract The study was designed with the aim to evaluate metformin and atorvastatin for its wound Healing activity.Two different formulations of Metformin emulgel and atorvastatin emulgel were formulated using Design expert 9.0 .The drugs were first screened for its solubility in various surfactant, co-surfactants and oils. The solvents having maximum solubility was selected for the microemulsion formulation. LAS (Linear Alkyl benzene Sulfonate), Span 80 and Almond oil were selected as Surfactant, co-surfactant and oil respectively. Micro-emulsion formulation was optimized using ternary phase diagram, globule size and percent transmittance. F1 and F43 formulations were optimized for Metformin and atorvastatin respectively. This optimized micro-emulsion was than utilize to form Emulgel. Lecithin and Sepineo P 600 was used as gelling agent for the formation of Emulgel. Emulgel was then evaluated for spreadability, viscosity and drug release.The optimized formula contained 3% sepineo P600 and 1% penetration enhancer i.e., Oleic acid. This optimized gel with good spreadability was further assessed for in-vivo wound healing activity using excision model. The results showed better wound healing for metformin gel compared to standard Betadine Ointment and Atorvastatin emulgel. Hence study concludes Metformin gel can be a promising therapy to treat Diabetic foot ulcer.

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