DEVELOPMENT AND IN VITRO CHARACTERIZATION OF NANOEMULSION AS ADJUVANT FOR THE ADMINISTRATION OF VACCINE USING A MODEL ANTIGEN
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2016, Vol 7, Issue 7
Abstract
The present study has investigated the development of oil in water type nanoemulsion (NE) for the incorporation of vaccine. NE act as adjuvant and potentiate the immunogenicity of antigen. The NE was formulated using plant essential oil, surfactant tween 80 and co surfactant ethanol by high energy emulsification method. The formulations were optimized for surfactant and cosurfactant ratio, Smix concentration and a ternary phase diagram was constructed to identify the area of NE formation. Formulations of NE1 to NE5 were prepared by sonication which are subjected to varying number of sonication cycles to get NE in 100nm – 200nm range and NE was subjected to short term and long term stability assessment. The NE was characterized in vitro for transparency, droplet size, zeta potential, pH, viscosity, conductivity, surface tension, fourier transform infrared spectroscopy. Transparent stable nanoemulsion (NE5) having mean droplet diameter of 84.23nm was obtained at 3:2 ratio of Smix,(10%) and the oil and surfactant ratio of 1:5 (v/v). No significant change in droplet diameter was observed after one week of storage at different temperatures. Surfactant concentration has been found to play a vital role in obtaining a stable nanosized emulsion. Further the NE5 was incorporated with pertussis toxin (PTAg-NE5) as a model vaccine and analysed for compatibility, size and stability. The PTAg-NE5 showed the incorporation of antigen with a slight increase in size of 99.42nm. The developed NE was found to be suitable for the incorporation of vaccine and thus could potentiate the immunogenicity of antigen.
Authors and Affiliations
R. Vijaya , G. Aswini , S. Madheshwaran
Keywords
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- EP ID EP159614
- DOI 10.7897/2230-8407.07783
- Views 84
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