DEVELOPMENT AND VALIDATION OF A BIOANALYTICAL METHOD FOR DETERMINATION OF QUETIAPINE FROM HUMAN PLASMA
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2013, Vol 4, Issue 2
Abstract
A simple high-performance liquid chromatographic (HPLC) method for the analysis of the antipsychotic drug quetiapine fumarate in human plasma has been developed. Zolpidem tartrate was employed as the internal standard (IS). Biological samples were pretreated by liquid-liquid extraction (LLE) technique using tert-butyl methyl ether (TBME). Separation was performed on a HiQSil C18HS (250x4.6 mm, 5µm) column. The mobile phase used was Acetonitrile-ammonium acetate buffer (pH 3.5, 10 mM) (40:60 v/v) pumped at a flow rate of 1 mL/min. Samples were injected by means of an autosampler via a variable loop and detected using UV detector at a wavelength of 254 nm. A good linearity was found in the concentration range of 100-2000 ng/mL. Within and between batch precision and accuracy of the proposed method were evaluated by percent relative standard deviation (% R.S.D.) and percent relative error (% RE) respectively; both being within the acceptable limits. The method was also validated for recovery, carry-over and stability. Freeze and thaw stability, short term stability and long term stability were evaluated for the developed bioanalytical method. The described method can be applied for quantitation of quetiapine in real clinical samples.
Authors and Affiliations
Vineeta Khanvilkar, Aditi Chitnis, Abhay Shirode , Vilasrao Kadam
Keywords
Related Articles
- EP ID EP146156
- DOI -
- Views 99
- Downloads 0
PRELIMINARY PHYTOCHEMICAL ANALYSIS OF PYRROSIA LANCEOLATA (L.) FARWELL
Pyrrosia lanceolata (L.) Farwell. was well-known as medicinal fern for skin disease and sore throat from ancient times in various literatures. There is no any report regarding the study of this plant earlier and hence it...
FORMULATION AND EVALUATION OF DENTAL FILM FOR PERIODONTITIS
Controlled release local drug delivery systems offer advantages compared to systemic dosage forms for periodontitis. The objective of this research was to design and evaluate sustained release dental films containing lom...
GLYCOGEN SYNTHASE KINASE 3Β PREDICTION AS PRIMARY CELLULAR TARGET TO MEDIATE ANTI-HEPATITIS C EFFECT OF NITAZOXANIDE
Hepatitis C is a major healthcare problem that launched the quest and the interest of scientists to search for solutions that alleviate its risks. New groups of drugs are developed and repurposed for this reason. One of...
PULSATILE DRUG DELIVERY SYSTEM: A REVIEW
The purpose for this review on pulsatile drug delivery systems (PDDS) is to compile the recent literatures with special focus on the different types and approaches involved in the development of the formulation. Pulsatil...
ASSOCIATION OF SERUM ALBUMIN CONCENTRATION AND PERIODONTITIS
Periodontal disease is a complex, multi-factorial, chronic inflammatory disease that involves degradation of periodontal structures, including alveolar bone. Many systemic diseases and disorders have been implicated as r...