Development and validation of a High performance liquid chromatography-Mass spectrometry method for 17 a -methyltestosterone in Aquatic water systems

Journal Title: Journal of Applied and Natural Science - Year 2010, Vol 2, Issue 2

Abstract

17 a -Methyltestosterone is used as feed additive to manipulate the gender of fish for aquaculture. Earlier a simple, yet specific and robust validated high performance liquid chromatographic method has been developed for the determination of 17 a -methyltestosterone in fish feed. The present work describes a highly sensitive and robust Liquid Chromatography-Mass Spectrometry method for the quantitation of 17 a -methyltestosterone in aquatic water systems using 17 β -hydroxy-3 β -methoxyandrost-5-en-7-one as internal standard. The method was validated in the concentration range of 0.2 to 25 ng of 17 a -methyltestosterone on column leading to a limit of quantitation of 0.08 ppb or 0.08 mg/L in water, and has potential to increase the limit of detection and quantitation by an order of magnitude, if required.

Authors and Affiliations

Ashok Marwah, Xu Nianzu, Padma Marwah, Terence Barry

Keywords

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  • EP ID EP126223
  • DOI 10.31018/jans.v2i2.117
  • Views 115
  • Downloads 0

How To Cite

Ashok Marwah, Xu Nianzu, Padma Marwah, Terence Barry (2010). Development and validation of a High performance liquid chromatography-Mass spectrometry method for 17 a -methyltestosterone in Aquatic water systems. Journal of Applied and Natural Science, 2(2), 182-189. https://europub.co.uk./articles/-A-126223