Development and Validation of a Preclinical Canine Model for Delayed Onset Fracture-Related Infections
Journal Title: Journal of Orthopaedic Science and Research - Year 2024, Vol 5, Issue 3
Abstract
Background: Fracture-Related Infections (FRIs) are a challenging complication in orthopaedics. FRI incidence is considerably high, particularly in open fractures. FRI management typically involves multiple surgical interventions and prolonged antibiotic therapies. This regimen is often ineffective at infection eradication, resulting in poor outcomes and inefficient use of healthcare resources such that improved preventative and therapeutic interventions are needed. To effectively address these gaps, valid preclinical animal models for FRIs are needed. The purpose of this study was to develop and validate a canine ulna model for delayed FRIs that accurately mimics the clinical course noted in patients. Methods: In this model, a distal ulnar ostectomy was established, then internally stabilized with plates and screws that were pre-incubated with methicillin-resistant Staphylococcus aureus. After a 3-week period, all animals underwent irrigation and debridement of the fracture site followed by clinical, radiographic, bacteriologic, and histologic assessments over the subsequent 8 weeks. Results: This preclinical canine model established a valid representation of delayed FRI in patients based on clinical, radiographic, bacteriologic, and histologic features. Bilateral distal ulnar ostectomies stabilized with MRSA-incubated implants were consistently associated with clinical signs of local infection, radiographic evidence for delayed union with osteomyelitis and implant failure, and implant-associated biofilm formation 3 weeks after “fracture” creation. Conclusion: The translational rigor of the model allows for efficient and effective testing of novel preventive and therapeutic interventions aimed at improving outcomes for FRI patients.
Authors and Affiliations
Bryce W Rigden1,2, Aaron M Stoker1,2, Chantelle C Bozynski1,2, Kyle M Schweser1,2, Tamara Gull3, Cristi R Cook1,2, Keiichi Kuroki3, James L Cook1,2*
Keywords
Related Articles
- EP ID EP753276
- DOI https://doi.org/10.46889/JOSR.2024.5314
- Views 9
- Downloads 1
Time and Travel Burden Associated with Hexapod-Assisted Correction of Lower Extremity Deformity
Background: Hexapod devices, colloquially referred to as frames, are powerful tools to correct lower extremity deformity. However, patients who undergo treatment with frames are followed closely to ensure that clinical a...
The Etiological Understanding of Spine Deformities in Patients with Neuromuscular Disorders
Purpose: The etiology understanding is the corner stone for proper management of the spine deformities in patients with neuromuscular disorders. Methods: Progressive spine deformities have been encountered in 71 patie...
How Sterile Are Our Operating Rooms? A Comparison of Air Quality During Primary TKA and THA
Introduction: Airborne Biologic Particles (ABPs) are a potential risk factor for infection following total joint arthroplasty. Factors such as temperature and humidity may affect the number of ABPs which can increase the...
Post-Operative Radiological Outcome of Hamstring Graft in ACL Reconstruction after Augmentation of Graft with Periosteal Envelope
Introduction: Anterior Cruciate Ligament (ACL) reconstruction with hamstring graft is usually done for the surgical management of ACL tear. The post-operative outcome of ACLR is mainly dependant on graft healing its in...
Morbidity and Total Knee Arthroplasty as a Long-Term Sequela of Tibial Plateau Fractures
Background: Tibial plateau fractures comprise a heterogenic group of patients, involving several mechanisms and variability in demographic characteristics. Although short term peri-operative clinical and radiologic outco...