Development And Validation Of A Rp-Hplc Method For Simultaneous Determination Of Cilnidipine And Chlorthalidone In Pure And Pharmaceutical Dosage Form
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2017, Vol 12, Issue 6
Abstract
A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of Cilnidipine and Chlorthalidone, in its pure form as well as in tablet dosage form. Chromatography was carried out on an Altima C18 (4.6 x 150mm, 5µm) column using a mixture of ACN, Methanol and Phosphate buffer pH4.6 (10:25:65 v/v) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 234nm. The retention time of the Cilnidipine and Chlorthalidone was 2.088, 6.068 ±0.02min respectively. The method produce linear responses in the concentration range of 25-125ppm of Cilnidipine and 10-50ppm of Chlorthalidone. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.
Authors and Affiliations
Hidayath unnisa, Parbati kirtania roy
Keywords
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- EP ID EP392181
- DOI 10.9790/3008-1206012869 .
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