DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF CLOPAMIDE, DIHYDROERGOCRISTINE MESYLATE AND RESERPINE IN TABLETS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 7
Abstract
Objective: To develop a new, rapid, precise and sensitive RP-HPLC method for the simultaneous determination of Clopamide (CPM), Dihydroergocristine mesylate (DHECM) and Reserpine (RSP) in tablets.Methods: Caffeine (CAFF) was used as internal standard, a BDS Hypersil column C8 (4.6 x 150 mm) with mobile phase containing a mixture of inorganic phase (ammonium acetate solution in water) and organic phase (acetonitrile and methanol), adjusted with phosphoric acid (10% v/v) to a pH-3.5, the flow rate was 2.0 ml/min, UV detection was performed at a wavelength of 220 nm and injection volume was 20 μl. The validation of this method was done as per ICH guidelines.Results: Retention times were observed as 1.277, 2.397, 3.799 and 5.202 min for Caffeine, Clopamide, Dihydroergocristine mesylate and Reserpine, respectively. Linearity ranges were observed to be: 1.50-300.00, 2.50-500.00 and 1.00-200.00 μg/ml for Clopamide, Dihydroergocristine mesylate and Reserpine respectively. Relative Standard Deviation did not exceed 2.00%. Detection limits were observed to be 0.08, 0.07 and 0.05 μg/ml for Clopamide, Dihydroergocristine mesylate and Reserpine, respectively.Conclusion: This method was applied, the first time as simple, rapid, precise and sensitive method for determination of Clopamide, Dihydroergocristine mesylate and Reserpine either alone or in combination, in pure form and in pharmaceutical preparations without interference with any excipient.Â
Authors and Affiliations
Hussen Al-akraa, Mohamad Kabaweh
Keywords
Related Articles
- EP ID EP578539
- DOI -
- Views 51
- Downloads 0
EXTRACTION OF FLAVONOIDS FROM BUCHANANIA LANZAN SPRENG. SEEDS BY SUPERCRITICAL FLUID EXTRACTION AND DETERMINATION OF THEIR ANTIOXIDANT ACTIVITY
Objective: The purpose of the study is to optimize supercritical fluid extraction (SFE) conditions for the flavonoids from the seeds of Buchanania lanzan Spreng. followed by quantitative determination of the antioxidant...
FAMILY ACANTHACEAE AND GENUS APHELANDRA: ETHNOPHARMACOLOGICAL AND PHYTOCHEMICAL REVIEW
Aphelandra belong to family Acanthaceae. We have reviewed traditional uses, pharmacological potential and phytochemical study of family Acanthaceae and genus Aphelandra. Traditionally the most important part use in Acant...
A VALIDATED RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF PYRANTEL PAMOATE AND PRAZIQUANTEL IN BULK AND PHARMACEUTICAL DOSAGE FORM
Objective: To develop a simple, accurate and precise reverse-phase high-performance liquid chromatography (RP-HPLC) method and subsequently validate for the simultaneous estimation of praziquantel (PZQ) and pyrantel pamo...
PARAMETRIC OPTIMIZATION OF FERULIC ACID ESTERASE PRODUCTION FROM MUCOR HIEMALIS NCIM 837PARAMETRIC OPTIMIZATION OF FERULIC ACID ESTERASE PRODUCTION FROM MUCOR HIEMALIS NCIM 837
Objective: To optimize the parametric conditions for the production of ferulic acid esterase from Mucor hiemalis NCIM837 using HPLC for quantification. Methods: The experiments were conducted to determine the influence o...
QUANTIFICATION OF SEED OIL AND EVALUATION OF ANTIOXIDANT PROPERTIES IN THE WILD AND CULTIVATED SPECIES OF SESAMUM L. (PEDALIACEAE)
Objective: Seed oil quantity and sesamin concentration in the wild and cultivated species of Sesamum from Kerala viz., Sesamum indicum, S. malabaricum, S. radiatum, S. laciniatum, S. prostratum and S. alatum were estimat...