DEVELOPMENT AND VALIDATION OF UV SPECTROPHOTOMETRIC ESTIMATION OF EBASTINE IN BULK AND TABLET DOSAGE FORM USING AREA UNDER CURVE METHOD
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2013, Vol 4, Issue 6
Abstract
The aim of this work is to develop a simple, accurate, reproducible and cost effective spectrophotometric method for determination of Ebastine in bulk and pharmaceutical dosage form. This method is basedon area under curve (AUC) in wavelength range of 247-257nm and method has followed linearity in the concentration range of 5-30μg/ml. Methanol was used as a solvent. The developed analytical method was validated as per International Conference on Harmonization (ICH) guidelines. The value of correlation coefficient (R2) was 0.999. Limit of Detection and Limit of Quantitation were calculated as 0.78µg/ml and 2.37µg/ml, respectively. Results of the recovery studies showed good accuracy of the method. Validation results suggest that the developed method can be used for routine quality control studies for assay of Ebastine in bulk and tablet dosage form.
Authors and Affiliations
Manish Dahivadkar , Hemant Jain , Kishore Gujar
Keywords
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- EP ID EP98663
- DOI 10.7897/2230-8407.04645
- Views 122
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