Development and validation RP-HPLC method for simultaneous estimation of Sulfamethoxazole and Trimethoprim
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2017, Vol 5, Issue 3
Abstract
A simple, economic, selective, precise, and accurate Reverse phase High Performance Liquid Chromatography method for analysis of Sulfamethoxazole and Trimethoprim, was developed and validated according to ICH guidelines. The quantification of drug was carried out using Agilent C18 250mm× 4.6mm × 5µm column or its equivalent in isocratic mode, with mobile phase compressing of Triethylamine: Acetonitrile (30:70) the flow rate was 1.0ml/min and the detection was carried at 260nm. The retention time and percentage assay for Sulfamethoxazole and Trimethoprim was found to be 2.688 and 4.388min, 100.23% and 99.30% respectively. Proposed method was validated for precision, accuracy, linearity range, specificity and robustness.
Authors and Affiliations
Gurumurthy. T, Monika. M, Ashwini. V
Keywords
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