Development of an Efficient Plant Regeneration System of Field Mustard (Brassica campestris)
Journal Title: Biotechnology Journal International - Year 2016, Vol 10, Issue 2
Abstract
Aims: The present study was conducted with a view to develop an efficient protocol for high frequency plant regeneration of Brassica campestris for further crop improvement program by biotechnological manipulation and to optimize this system for regeneration of a number of B. campestris genotypes. Study Design: Completely Randomized Design. Place and Duration of Study: This experiment was carried out in the Genetic Engineering Laboratory of the Department of Genetics and Plant Breeding, Sylhet Agricultural University, Bangladesh during the period of July 2013 to June 2014. Methodology: Cotyledon and hypocotyl explants of B. campestris cv. BARI sarisha-12 were cultured on MS medium supplemented with different concentrations of 6-Benzylaminopurine (BAP) and α-Naphthaleneacetic acid (NAA) for callus initiation and shoot regeneration. Later on subsequent subculturing is done for shoot elongation and multiplication. MS medium supplemented with various concentrations of NAA were used for root formation. Results: From a total of 15 different combinations of BAP and NAA tested, the combination of 1.0 mg L-1 BAP and 0.5 mg L-1 NAA gave the highest frequency of callus initiation (94.44%) as well as shoot regeneration (63.89%) in case of cotyledon explants where as hypocotyl explants showed 47.62% callus initiation and 19.04% shoot regeneration frequency. Four days old cotyledon explants showed the highest shoot regeneration frequency (72.22%) and higher number of shoots per explant (3.94) than those from older seedling. The shoot regeneration frequency markedly enhanced to 83.33% by the addition of 2.0 mg L-1 AgNO3 to the MS medium supplemented with 1.0 mg L-1 BAP, 0.5 mg L-1 NAA and this combination also showed the maximum number of shoots per explant (6.86). Shoot regeneration potentiality of five B. campestris genotypes were investigated and indicated that this system would be widely applicable to all the genotypes. The regenerated shoots were easily rooted on MS medium supplemented with 0.2 mg L-1 NAA and the whole plants were transferred to pot soils and grown to maturity. Conclusion: MS medium supplemented with 1.0 mg L-1 BAP, 0.5 mg L-1 NAA and 2.0 mg L-1 AgNO3 is more efficient for multiple shoot regeneration by using cotyledon explants and it may be utilized for In vitro improvement program of B. campestris.
Authors and Affiliations
Mohammed Shafi Ullah Bhuiyan, Kishore Kumar Sarker, Fakhrul Islam Monshi, Sayeda Sultana, Delara Akhter
Keywords
Related Articles
- EP ID EP237684
- DOI 10.9734/BBJ/2016/21529
- Views 100
- Downloads 0
Phytochemical and Antimicrobial Screening of Root Extracts of Dacryodes edulis
Aims: To screen extracts of Dacryodes edulis phytochemically. To assay the said extracts for antimicrobial activity. To validate or otherwise claims by traditional medicine practitioners on Dacryodes edulis. Place and Du...
In vitro Propagation and GC-MS Studies of Ocimum basilicum Linn. var. pilosum (Willd.) Benth
An in vitro propagation method is outlined for Ocimum basilicum Linn. var. pilosum (Willd.) Benth., a wild aromatic plant belongs to Lamiaceae family. Shoot buds were used as source of explants on MS media supplemented w...
Constraining the Pluripotent Fate of Human Embryonic Stem Cells for Tissue Engineering and Cell Therapy – The Turning Point of Cell-Based Regenerative Medicine
To date, the lack of a clinically-suitable source of engraftable human stem/progenitor cells with adequate neurogenic potential has been the major setback in developing safe and effective cell-based therapies for regener...
Effect of kLa and Fed-batch Strategies for Enhanced Production of Xylitol by Debaryomyces nepalensis NCYC 3413
Aims: To evaluate the effect of volumetric oxygen transfer coefficient on the production of xylitol by Debaryomyces nepalensis and to enhance the yield and productivity of xylitol by fed-batch fermentation using xylose a...
Antimicrobial Activities of Essential Oils from Some Medicinal and Aromatic Plants
This research work was designed to examine the antimicrobial activities of some medicinal and aromatic plants. The essential oils of Adansonia digitata, Arachis hypodaea, Capsicum frutecens, Gossypium hirsutum, Hisbiscus...