Development of approaches to standardization of Helichrysum arenarium (L.) Moench. in multicomponent plant composition
Journal Title: Фармацевтичний журнал - Year 2014, Vol 6, Issue 6
Abstract
Determined marker for qualitative and quantitative standardization of Helichrysum arenarium (L.) Moench. flowers in the plant mixtures. As a marker of Helichrysum arenarium (L.) Moench. flowers can be used apigenin. Apigenin has a wide range of biological activity (in particular, apigenin has anti-inflammatory, antioxidant, anti-cancer and many other pharmacological properties). The method for the analysis of apigenin in Helichrysum arenarium (L.) Moench. flowers and its herbal mixtures using HPLC was developed. Chromatographic study investigated extracts and reference solution of apigenin was carried out by chromatograph Shimadzu ser. 20, equipped with a diode array detector under the following conditions: column Phenomenex Luna C18 (2) (250 mm × 4.6 mm, 5 micron particle size), the column temperature – 35 °C, detection wavelength – 330 nm, the flow rate of the mobile phase – 1ml/min, volume injection – 5mcl. As the mobile phase used a 0.1% solutions of trifluoroacetic acid in a mixture of water and acetonitrile (gradient elution). With the developed methodology analyzed of 5 series Helichrysum arenarium (L.) Moench. The analysis shows, that the content of apigenin in raw plant is between 0,1453 ± 0,0068% to 0,1657 ± 0,0085% in terms of the dried materials. It is shown, that the presence and content of apigenin Helichrysum arenarium (L.) Moench. flowers can be standardized in the mixtures with the following plant: leaves and fruits of genus Crataegus L., roots of Althaea officinalis L., roots of Glycyrrhiza glabra L., roots of Cichorium intybus L., roots of Taraxacum officinale Webb., cones of Humulus lupulus L., grass of Leonurus cardiaca L., grass of Hypericum perforatum L. and seeds of Linum usitatissimum L.
Authors and Affiliations
A. V. Gudzenko
Keywords
Related Articles
- EP ID EP591721
- DOI -
- Views 67
- Downloads 0
Determination of the list of narcotic analgesics for providing population in conditions of emergency situations
The current state of natural, technological and socio-political safety of Ukraine determines the urgency and necessity of improvement the efficiency of the pharmaceutical supply of the population at liquidation of conseq...
Antibiofilm activity of aminopropanol derivatives against Pseudomonas aeruginosa
Bacterial biofilm, particularly formed by Pseudomonas aeruginosa, are a cause of severe chronic infectious diseases. Bacteria within a biofilm are phenotypically more resistant to antibiotics and the macroorganism immune...
Structural and adsorptive properties of silica-alumina and clay materials of mineral and synthetic origin
Location on territory of Ukraine of great deposits of aluminosilicates together with good facilities for their processing and standardization promotes the study of this raw material, results of which could be useful for...
Scientific and methodological principles of teaching the history of pharmacopoeia’s creation for students of pharmaceutical faculty
Teaching students of pharmaceutical faculty the history of medicines, including the development and implementation of Pharmacopoeia in the medical and pharmaceutical practice, isessential for the development of the irpos...
Study of extraction efficiency of prenylated flavonoids from hops raw (Humulus lupulus L.) by the ethanol containing extraction solvents
Development of technology of the hop dry extract as APIs provided for a detailed study and identification of factors that can affect the quality characteristics of the finished product. The goal of this study was to inv...