Development of Validated RP-HPLC Method for Simultanious Estimation of Cefadroxil and Ambroxol in Pure and Pharmaceutical Dosage Form
Journal Title: Journal of Pharmaceutical Research International - Year 2017, Vol 15, Issue 6
Abstract
A precise, accurate, economical and simple HPLC (reverse phase) method was developed for the estimation of Cefadroxil and Ambroxol in bulk and pharmaceutical dosage form. Method was performed on a octadecyl silane column with dimensions 4.6 x 250 mm having particle size 5 micron. The mobile phase used in the method is methanol: phosphate buffer pH 3 (60:40v/v). The rate of flow was maintained at 1.5 ml/ min and effluent was monitored at 243 nm. Retention times were observed 2.3 min and 3.3 min for CFX and AXL respectively. The standard calibration curve was found linear over a range of 16–80 µg/ml and 2-10 µg/ml for CFX and AXL respectively. Similarly an average correlation coefficient was also obtained at 0.999 for CFX and AXL. LOQ limit was found at 2 µg/ml and absolute recovery was found 99.6% and 99.5% for CFX and AXL respectively. This method can be utilized for routine analysis of CFX and AXL in pure and dosage form.
Authors and Affiliations
Tribhuvan Singh, Dhiraj Kumar, Anurag Mishra, Sandeep Arora, D. V. Kishore Kumar, Sagar Pamu, Himansu Bhusan Samal, Velupula Nishanth
Keywords
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- EP ID EP312979
- DOI 10.9734/BJPR/2017/32225
- Views 119
- Downloads 0
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