DEVELOPMENT, STANDARDIZATION AND VALIDATION OF HPLC METHOD FOR ESTIMATION OF βSITOSTEROL IN VITEX NEGUNDO SEED EXTRACT
Journal Title: Int J of Pharm Rev& Res - Year 2015, Vol 5, Issue 2
Abstract
A simple, precise and accurate high-performance liquid chromatographic method has been developed for determination of β-sitosterol in the hydroalcoholic extact of Vitex negundo Linn. A Reverse phase HPLC column CrestPack RP C18 (Make: Jasco; 250 mm x 4.6 mm I.D; particle size 5μm) was used as stationary phase with a mobile phase comprising of acetonitrile : methanol (70:30) v/v at a flow rate of 1.0 mL min-1 and UV detection at 210nm with a run time of 6 mins. The proposed method was validated for linearity, accuracy, precision, LOD and LOQ. LOD for beta-sitosterol was found to be 0.5μg/mL whereas LOQ for the same was found to be 1.5μg/mL. The method was found to be linear over the range 1 μg/mL to 5μg/mL with coefficient of regression 0.9732 for betasitosterol. Intra-day and inter-day precision studies showed % RSD to be less than 2 and hence the method was precise. Stock solution stability studies were carried out for beta-sitosterol. Stability samples in methanol after a week revealed that all beta-sitosterol in solution form were stable. The validated HPLC method can be used for a routine quality control analysis and quantitation of β-sitosterol from Vitex negundo Linn.
Authors and Affiliations
Divya K Shetty , Swati S Patil
Keywords
Related Articles
- EP ID EP106564
- DOI -
- Views 160
- Downloads 0
INSILICO COMPARATIVE STUDY OF VIRAL GENOMES OF PARVOVIRIDAE
The Current Research deals with comparative study and functional similarities of viral genomes of the family parvoviridae. Parvoviridae is a viral family of single stranded DNA viruses with some identical proteins for th...
AN UPDATED REVIEW ON ANTI-ARTHRITIC MEDICINAL PLANTS
Arthritis is a chronic, inflammatory, systemic autoimmune disease characterized by pain, swelling and stiffness. Allopathic medications have been prescribed to alleviate symptoms of this disease which results into associ...
IN VITRO STUDY ON THROMBOLYTIC ACTIVITY OF HYDROALCOHOLIC EXTRACT OF VALERIANA JATAMANSI AND ORIGANUM VULGARE LEAVES
The present study was investigated to find out the thrombolytic activity of hydroalcoholic extract of Valeriana Jatamansi and Origanum Vulgare leaves by using in vitro method along with streptokinase as a standard. The s...
FORMULATION AND EVALUATION OF NEVIRAPINE LOADED CHITOSAN AND EUDRAGIT NANOPARTICLES FOR ANTIRETROVIRAL THERAPY
The goal of the present investigation was to formulate and evaluate chitosan and Eudragit® nanoparticles of nevirapine for antiretroviral therapy. Nanoparticles of nevirapine were prepared using chitosan, Eudragit® S 100...