Diagnosis of contagious ecthyma in goats in a quarantine station in Panama
Journal Title: Revista MVZ Cordoba - Year 2014, Vol 19, Issue 3
Abstract
We report an outbreak of contagious ecthyma (CE) in a herd of goats at Paso Canoas quarantine station, Panama. The goats were adult intact females. Visible clinical signs became apparent from day 13 after the start of quarantine. We performed clinical examination. Serum biopsy and scabs were collected from crusted lesions in the epithelium of the lips, nose and eyelid corners. Samples were studied by histopathology,complement fixation test, transmission electron microscopy (TEM), DAS-ELISA, viral isolationand nucleic acid amplification tests. Histopathology revealed ortho and parakeratotic hyperkeratosis, epithelial hyperplasia, viral inclusion bodies, keratinocytes with balonoid degeneration, vesicles with neutrophils and degenerated cells, in superficial dermis there is marked neovascularization. Complement fixation test, DAS-ELISA and nucleic acid amplification tests resulted positive for contagious ecthyma. TEM showed viral particles, consistent with Parapoxvirus. Clinical and laboratory findings were consistent with poxvirus infection in the quarantine goat herd.
Authors and Affiliations
Angie Magaña Ch, MMVZ *| Ministry of Agricultural Development, National Directorate of Animal Health, Laboratory of Diagnosis and Veterinary Research Dr. Gerardino Medina H., Río Tapia, Tocumen, Edificio LADIV, Panamá, Panamá.Correspondence: patovetpanama@gmail.com, Nathaniel Kadoch Z, M. Sc| Ministry of Agricultural Development, Executive Management of Agricultural Quarantine, Altos de Curundu, Calle River, Building 577, Panamá, Panamá., Anarvik Sánchez P, M. Sc| Ministry of Agricultural Development, Laboratory of Vesicular Disease Diagnosis, Río Tapia, Tocumen, LADIVES Building, Panamá, Panamá., César Maure M, MV| Ministry of Agricultural Development, Executive Management of Agricultural Quarantine, Altos de Curundu, Calle River, Building 577, Panamá, Panamá., Rolando Tello J, M. Sc| Ministry of Agricultural Development, Executive Management of Agricultural Quarantine, Altos de Curundu, Calle River, Building 577, Panamá, Panamá.
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