Dissecting the underlying pharmaceutical mechanism of Danggui Buxue decoction acting on idiopathic pulmonary fibrosis with network pharmacology
Journal Title: Traditional Medicine Research - Year 2020, Vol 5, Issue 4
Abstract
Backgroud: Danggui Buxue decoction (DBD), a classical prescription in traditional Chinese medicine, has been found to have protective effect on bleomycin-induced pulmonary fibrosis in rats by reducing alveolar inflammation and fibrosis. However, the biological activity of individual chemical components and mechanism of action of whole formula are not clear. Methods: Potential targets of active ingredients of DBD were collected through Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform and SymMap database. Target genes related to idiopathic pulmonary fibrosis were obtained from the Online Mendelian Inheritance in Man database, Therapeutic Targets Database and Gkb database. Then, the common targets were obtained by overlapping the potential targets of active ingredients in DBD and diseases related targets. The selected targets were subjected to Kyoto Encyclopedia of Genes and Genomes signaling pathway and Gene Ontology analysis, and the network map of active component-target-pathway was established using Cytoscape 3.7.1 software. The active components of DBD with most targets were selected for fibrosis-related marker verification. The mRNA and protein expression of fibrosis markers, α-smooth muscle actin, collagen 1 and fibronectin, were detected in TGF-β1-induced fibroblast cell line after treatment with the active components. Results: The 14 active ingredients, such as quercetin and kaempferol, were screened from DBD. It acts on 26 targets like estrogen receptor 2 and prostaglandin-endoperoxide synthase 2, and mainly involves 38 signaling pathways such as cell inflammation and autophagy. Kaempferol and quercetin are the two compounds with the highest network regulation, which can inhibit the transformation of fibroblasts into myofibroblasts and reduce the expression of fibrosis markers α-smooth muscle actin, collagen 1 and fibronectin. Conclusion: The integration mode of multi-component, multi-target, multi-channel and mechanism of DBD in the treatment of idiopathic pulmonary fibrosis are predicted by means of network pharmacology. Our study could indicate the direction of further anti-fibrotic mechanism research.
Authors and Affiliations
Cai-Ping Zhao,Hang Li,Xiao-Hong Liu,Shuang Liang,Xue-Lei Liu,Xin-Rong Li,Yi Luo,Mei-Ling Zhu
Keywords
Related Articles
- EP ID EP683789
- DOI 10.12032/TMR20191102146
- Views 212
- Downloads 0
Effect of Ganduqing Granule on metabolic disorder and liver dysfunction in patients with chronic hepatitis B
Objective: This study investigated the potential mechanisms of Ganduqing Granule (GDQG) in improving the liver function in patients with chronic hepatitis B (CHB). Methods: Plasma samples from 30 healthy volunteers and 3...
Traditional Chinese medicine should not be ignored during the development of precision medicine with Chinese characteristics
TCM has been playing an important role in disease treatment for thousands of years. This study presents that it may contribute to the development of precision medicine in China, if the prevention, diagnosis and treatment...
Study on the relationship between the structure of bacterial flora on the tongue and types of tongue coating in patients with type 2 diabetes mellitus
Highlights The incidence of type 2 diabetes mellitus (T2DM) is related to the imbalance of oral microflora in the human body. The characteristic tongue coating flora structure, containing Prevotella, Fusobacterium, St...
The dynamic changes and mechanisms of Rehmanniae radix processing based on Maillard reaction
Background: Traditional Chinese medicines are usually processed before they are used for clinical treatment. This is done in a way associated with the Maillard reaction. Methods: Based on the Maillard reaction, this pape...
Bibliometric analysis of acupuncture research through the Web of Science database from 1990 to 2019
Background: The main points of focus of bibliometric analysis of acupuncture treatment of diseases include pain (headache, low back pain), insomnia, and knee osteoarthritis, for example. In this paper, we analyze the fro...