DNA Polymorphism and Genetic Variation among Bivoltine Silkworm (Bombyx mori L.) Genotypes Revealed by RAPD Markers
Journal Title: Biotechnology Journal International - Year 2017, Vol 20, Issue 4
Abstract
Background: In breeding programs, the selection of parental lines based on their phenotypic traits along with genetic variation at their DNA level is the better option in directional breeding as specific DNA profiles would be useful in producing reliable estimates of genetic identity and diversity at their genomic levels. In this context, Random amplified polymorphic DNA (RAPD) marker approach was used to ascertain genetic diversity and relationship among 12 bivoltine silkworms (Bombyx mori L.) genotypes for the identification of diverse genotypes to be used as parents for the improvement of quantitative traits in silkworms. Results: RAPD analysis with 12 silkworm genotypes revealed high polymorphism of 88.88%. Ten RAPD primers utilized in this study generated PCR products in the range of 202-2876 bp across 12 silkworm genotypes. The Polymorphism information content (PIC) ranged from 0.295 to 0.493%. Based on Nei’s similarity coefficients, the genetic distance among the studied silkworm genotypes ranged from 0.93 to 0.99. Of the pairwise combinations, CSR18 and SH6, showed the highest similarity (0.99) whereas, SKAU-R-6 and DUN6 showed the lowest similarity (0.93) among the genotypes. The dendrogram generated, using Unweighted pair group method using the arithmetic average (UPGMA) from this marker, grouped the 12 silkworm genotypes into five main clusters, which corroborates the differences in their phenotypic characters. Conclusion: The study clearly indicated the potential use of RAPD markers in differentiating silkworm genotypes based on their characters. The information generated on genetically divergent genotypes identified in this study would be useful in future silkworm breeding programmes for modifying the yield potentials of silkworms so as to boost up bivoltine silk production in the interest of temperate sericulture industry.
Authors and Affiliations
Z. I. Buhroo, N. A. Ganai, M. A. Malik, A. S. Kamili
Keywords
Related Articles
- EP ID EP315079
- DOI 10.9734/BJI/2017/35046
- Views 112
- Downloads 0
Prevalence of Dyslipidemia on the Basis of Biochemistry Tests in the Parakou University Teaching Hospital (Benin)
Aims: Determine the prevalence of dyslipidemia in patients attended in the Parakou University teaching Hospital Biochemistry Laboratory (Republic of Benin). Study Design: Cross-sectional retrospective study. Place and D...
Alteration in Trace Elements Concentration and Possible Copper Toxicity in Juiced Leafy Vegetables Commonly Consumed in Southwestern Nigeria
This study investigated the effects of juicing on four micro-minerals (Cu, Zn, Mn and Mo) contents of six different leafy vegetables commonly consumed in South western, Nigeria. Micro-minerals were determined using Atomi...
Chemical and Amino Acid Composition of Raw and Defatted African Mango (Irvingia gabonensis) Kernel
The proximate, mineral, functional properties, anti-nutrients and amino acid compositions of African mango (Irvingia gabonensis) seeds have been investigated. The results showed that seeds contained ash (2.49 g/100g), mo...
Plant Regeneration via Somatic Embryogenesis in an Ethnomedicinal Plant Senna alata (L.) Roxb.
Aim: To study the effect of various plant growth regulators (PGRs) for induction of somatic embryogenesis and plantlet regeneration from cotyledon and leaflet explants in Senna alata (L.) Roxb. (Syn. Cassia alata) an imp...
Impact of High Glycine Tyrosine KAP Genes on Cashmere Fibre Trait Characteristics
Cashmere goat (Capra hircus) represents a world renowned goat famous for production of superfine downfibre “Cashmere”. The worldwide total production of cashmere fibre is about 10000-15000 tonnes per annum. Among all the...