ECONOMIC MAXIMIZATION OF ALFALFA ANTIMICROBIAL EFFICACY USING STRESSFUL FACTORS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 9
Abstract
Objective: The present study addresses the effect of water deficit stress on the antimicrobial capacity of alfalfa (Medicago sativa) plants. Methods: Methanolic extracts of alfalfa plants grown in different soil types, varying in sand proportion, either alone or combined with various levels of water regimes were assessed for antibacterial and antifungal activities following cup plate method. The phytochemical profiles of plant extracts were also qualitatively screened using appropriate chemical reagents. Moreover, data were intensively processed via two different statistical designs. Results: Increasing sand amount induced the inhibitory effect of plant extracts on Escherichia coli, Klebsiella pneumonia, Proteus vulgaris, Salmonella typhi, Mucor circinelloides, Rhizopus azygosporus and R. microsporus with less pronounced action on Shigella flexneri, Staphylococcus epidermidis, Candida albicans and Emericella quadrillineata; as well as a reversed influence on Pseudomonas aerugenosa and Streptococcus pyrogenes. Furthermore, withholding irrigation water enhanced the plant suppressive action on E. coli, Salmonella typhi, Staphylococcus epidermidis, Candida albicans and R. microsporus with less marked or reversed influence on the other tested microbes. However, Pseudallescheria ellipsoidea, two species of Penicillium and five of Aspergillus could resist the studied plant extracts. The results also revealed that the extracts of water-unsatisfied plants generally contained higher amounts of alkaloids, amino acids, flavonoids, glycosides, phytosterols, saponins, steroids, tannins, terpenoids and reducing sugars.Conclusion: The employed biological evaluations point out to promising antimicrobial efficiency of alfalfa plants particularly when stressed.
Authors and Affiliations
Bardees Mickky, Muhammad Abbas, Omar El-shhaby
DIFFERENTIAL EXPRESSION OF NITRIC OXIDE SYNTHASES IN THE SKIN TISSUE OF P-PHENYLENEDIAMINE-TREATED MICE
Objective: p-Phenylenediamine (PPD) is a possible contact sensitizer in skin tissues; however, data are lacking regarding its specific effects on nitric oxide synthase (NOS) expression during the sensitization phase. The...
ACUTE AND SUB-CHRONIC (28 DAYS) REPEATED ORAL TOXICITY TEST OF ETHANOL EXTRACT OF LERAK (SAPINDUS RARAK. DC) FRUITS IN WISTAR RATS
Objectives: Natural product which is used as medicines should in compliance with the guidelines that the drug does not cause acute or chronic toxicity and proved efficacious as a medicinal. The present study was carried...
EVOLUTION OF NANOTECH ASSISTED PCR DIAGNOSIS OF MYCOBACTERIUM TUBERCULOSIS AND ITS ASSESSMENT WITH CONVENTIONAL METHODS
Objective: This study was focused on assessment and performance of conventional and nanotech assisted methods for detection of Mycobacterium tuberculosis. Tuberculosis (TB) still leftovers one of the top ten causes of de...
BEHAVIORAL STUDIES OF WISTAR RATS IN ROTENONE INDUCED MODEL OF PARKINSON’S DISEASE
Objective: The objective of the study was to determine the behavioral activities of Wistar rats induced with rotenone. Methods: Thirty-six male Wistar rats were taken for the study and divided into six groups of six rats...
STRUCTURE-BASED DESIGN OF NOVEL RILPIVIRINE ANALOGUES AS HIV-1 NON-NUCLEOSIDE REVERSE TRANSCRIPTASE INHIBITORS THROUGH QSPR AND MOLECULAR DOCKING
Objectives: The aim of this research is to investigate the better biological activities from Rilpivirine analogues based on their Quantitative Structure-Property Relationship (QSPR) and pharmacophore study.Methods: In th...