Effect of 2,4-D and 2ip hormones on embryogenesis callus production and the effect of sucrose and concentrations of MS salts on somatic embryogenesis of date palm (cv. Medjool)
Journal Title: International Journal of Farming and Allied Sciences - Year 2014, Vol 3, Issue 11
Abstract
Somatic embryogenesis is one of the most common and effectual method for in vitro regeneration of date palms. In this study, production of embryogenesis callus in Medjool cultivar on high concentrations of 2,4-D (10, 30, 50, and 70 mg/l) with 2ip (0 and 3 mg/l) were examined. In the absence of 2ip hormone and the treatment with 70mg/l 2,4-D hormone, we witnessed the highest embryogenesis callus production of (2.68g) and production of embryogenesis calluses of about 2.57g when we used 3mg 2ip hormone with 50mg 2,4-D, accounted as the second order of production. Moreover, as we aimed at producing somatic embryos, embryogenesis calluses were exposed to two concentrations of 30 and 60mg/l sucrose and three different concentrations of MS salts, by which it was determined that the higher concentration of sucrose was applied, the more the number of embryos were produced. It was also revealed that for producing somatic embryos, 1/2MS and 3/4MS were more efficient than absolute MS.
Authors and Affiliations
Behnaz Abdolvand, Reza Zarghami, Hasan Hasani, Mohsen Mardi and Hedayat Zakki Zade
Keywords
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