Effect of Agaricus Sylvaticus (Schaeffer) Extract in Rats Skin Wound Healing
Journal Title: Biomedical Journal of Scientific & Technical Research (BJSTR) - Year 2018, Vol 10, Issue 1
Abstract
Mushrooms are an excellent food option for diets, since they are nourishing and not fattening, mainly due to their nutritional characteristics. They have a high protein content, low lipid values, besides the presence of vitamins (thiamine, riboflavin, niacin), minerals (calcium, iron, phosphorus), beta-glucans and antioxidant compounds. Several people are using it in Brazil as a blood glucose level controller and Anvisa released the Agaricus blazei as a nutraceutical product. In the present project the objective was to evaluate the healing of open cutaneous wounds in rats treated with topical use of Agaricus sylvaticus extract 10% in gel by macroscopic analysis of the cicatricial process. For skin healing of wound in rats, a total of 15 adults male Wistar rats (UNINOVE Vivarium) were used, divided in three groups. Animals in group 1 were treated with 1mL of gel at 10% Agaricus; group 2 with 1mL of distilled aqueous gel and group 3 with fibrinase. Daily applications were performed over the wound of total 4cm2 area in dorsal region of each animal. The wound evaluation was made macroscopically in the time stamps of 0, 7, and 14 days, and the wound evolution, and skin healing retraction measures evaluated by digital planimetry. Results indicated that extract from Agaricus presented proteins, phenols and beta glucan. As phenols chlorogenic, cafeic, coumaric and benzoic acid were presented and provided antioxidant reaction. The animals treated with Agaricus showed increased healing when compared with control and fibrinase treatment. After 14 days, the wounds in animals treated with Agaricus were healed corresponding to 73.79% of control group, while animals with fibrinase corresponded to 56,58% of control group. This demonstrates that fibrinase was not as efficient as Agaricus and when compared to control group. Thus, since Agaricus has an antioxidant factor and acted as a healing agent, it can be stated that the presence of phenols in the extract was important. It is possible to conclude that the gel with Agaricus is effective at skin healing in rats. Mushrooms have been used as a food source since ancient times, due to the believe of its high nutritional value and medicinal potential and were considered a noble ingredient in culinary dishes. Approximately 2000 edible species are known and about 25 of them are commercially grown. Mushrooms are an excellent food source for diets, since they are nourishing and not fattening, mainly because of their nutritional characteristics. They have high protein content (27-48%), low lipid values (2-8%), and vitamins (thiamine, riboflavin, niacin), minerals (calcium, iron, phosphorus), beta-glucans and compounds with antioxidant activity [1]. Few things are known about the quality of edible mushrooms grown in Brazil, about its nutritional or medicinal value. The data found on international literature are scare and the cultivation conditions are different from those found in Brazil [2]. Brazil has an immense and unexplored microflora. Wild species have untapped and unknown potential, both from the point of view of production and consumption, as well as secondary metabolites and their biological activity [3, 4]. In Brazil, we have ANVISA (National Agency of Healthy Surveillance-Brazil) which released for sale only powder of mushrooms for diet or as nutritional [5]. Currently, Agaricus sylvaticus (Schaeffer), Agaricus blazei (Murril) and Agaricus brasiliensis S. Wasser are similar names. The beta glucan was one of Agaricus’ extracted compounds, which is a polysaccharide polymer naturally found as the largest component of fungi cell wall [6]. Agaricus extracts were found in the laboratory concentrations above 200mg of beta glucan in addition to proteins and evaluated as an antidiabetic [7] and antitumor effect [4]. About healing that is a process by which an injured tissue is replaced by vascularized connective tissue, whether the lesion has been traumatic or necrotic [8]. Despite the predominance of synthetic substances in the therapeutic arsenal, including anti-inflammatory substances, over the years there has occurred the valorization of complementary therapeutic practices like the use of medicinal herbs. Several tests using phytotherapics in the healing process of skin lesions were promising. For example, Aloe Vera (mucilage) was effective in the treatment of cutaneous wounds in normal and diabetic rats [9]. The topical use of the 2% papain solution also showed good results in this wound in rats [10]. Compounds from mushrooms have also been explored as hypoglycemic, antitumor among others, but nothing has been published about its healing action. The aim of the present study was to evaluate the effect of Agaricus sylvaticus (Schaeffer) extract 10% gel on the healing of surgical skin wounds in rats using macroscopic analyses.
Authors and Affiliations
Gildiney Raimunda da Silva, Victor Franklin, Joseanne Meira Cambui, Danilo Tenorio de Almeida, Nilsa Sumie Yamashita Wadt, Vinicius O Cardoso, Erna Elisabeth Bach
Keywords
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- EP ID EP587843
- DOI 10.26717/BJSTR.2018.10.001903
- Views 164
- Downloads 0
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