EFFECT OF ANIONIC FLOCCULANT AND PH CHANGE ON THE FLOCCULATION EFFICIENCY OF PARACHLORELLA KESSLERI
Journal Title: European Journal of Biomedical and Pharmaceutical Sciences - Year 2017, Vol 4, Issue 12
Abstract
Microalgae have shown a great potential in the biotechnology field, however, high production costs have limited industrial applications. Biomass harvest is one of the major difficulties in microalgae cultivation due to high energy inputs which are needed to separate the cells from culture broth. Flocculation is considered to be a reliable method to improve cost-effectiveness in the down streaming processing. The effects of flocculation efficiency on harvesting Parachlorella Kessleri cultivated in 24 l photobioreactor were investigated by changing culture medium pH value and adding polyacrylamide flocculant (Magnafloc® 10). The flocculation experiments were conducted in 40 ml test tubes using culture broth obtained from the photobioreactor. The flocculation efficiency was evaluated by comparing the remaining cell density in the supernatant with the cell concentration before flocculation. The flocculation efficiency of 90 and 79% was obtained without flocculants and conducted under dark conditions for 24 hrs at 22ºC and 4ºC, respectively. For only 4 hrs settling time, 80% of flocculation efficiency was achieved by adjusting the culture pH to 10 using sodium hydroxide (NaOH) and adding Magnafloc® 10. By adjusting only pH value of the culture medium to 4 using Hydrogen Chloride (HCl), the measured flocculation efficiency was 97%. The addition of Magnafloc® 10 improved cell viability and slightly increase the flocculation efficiency. The anionic flocculant is only efficient when the pH of the cell culture was adjusted to a pre-defined value that induces cells immobilization and surface charge neutralization preceding flocculation process.
Authors and Affiliations
Youssef MIR
Keywords
Related Articles
- EP ID EP630431
- DOI -
- Views 142
- Downloads 0
FORMULATION DEVELOPMENT AND EVALUATION OF OXYMORPHONE HCL SOFT GELATIN CAPSULES
The objective of this work is to develop simple, elegant, liquid-filled immediate release soft gelatin capsules containing Oxymorphone HCl intended for the management of moderate to severe pain in patients who require tr...
MORPHOMETRIC EVALUATION OF THE EXTERNAL EAR IN RELATION TO ATTACHMENT PATTERN: A STUDY OF ADULT NIGERIANS RESIDENT IN THE SOUTHERN REGION
There are two configurations of earlobe in humans; attached and unattached earlobe. This study was done to evaluate the morphology of the external ear in relation to attachment pattern and to determine the association be...
PRESCRIPTION PATTERN IN PATIENTS WITH CORONARY ARTERY DISEASE IN A TERTIARY CARE HOSPITAL: A SYSTEMIC REVIEW
Introduction: Impedance or blockage of one or more arteries which supply blood to the heart, usually due to atherosclerosis. Abbreviated CAD, A major cause of illness and death, CAD begins when the hard cholesterol subst...
THE TIME AND DOSE DEPENDENT EFFECTS OF HALOFANTRINE HYDROCHLORIDE ON THE BIOCHEMICAL LIVER INDICES OF GUINEA PIG
Halofantrine hydrochloride is an antimalarial drug, usually prescribed as a standby treatment of malaria in travellers and pregnant mothers. In this study the time and dose dependent effects of halofantrine hydrochloride...
ASSESSMENT OF DELIVERY OUTCOMES AND FACTORS ASSOCIATED WITH LOW BIRTH WEIGHT FOLLOWING THE INTRODUCTION OF SP-IPTP AS PART OF FOCUSED ANTENATAL CARE IN THE HOHOE MUNICIPALITY, GHANA
Background: Intermittent Preventive Treatment with Sulphadoxine-Pyrimethamine (SP-IPTp) is a key component of malaria control in pregnancy. This study assessed delivery outcomes and factors associated with low birth weig...