Effect of Carbomer as an Adjuvant for Enhancement of Immune-Response Against FMD Vaccine
Journal Title: Journal of Applied Veterinary Sciences - Year 2021, Vol 6, Issue 2
Abstract
This work was designed to provide high protective, long-lasting immunity against FMD by enhancing the immunogenicity of the trivalent FMD vaccine using carbomer as adjuvant using G. pigs as an alternative cheapest animal model for quality control testing of the prepared FMD vaccines formulae. Guinea pigs were chosen as experimental models to develop concepts and techniques to study the PD50 of FMD vaccines because of the similarities of clinical symptoms in these animals to those of swine and cattle to saving cost, three different formulae of inactivated trivalent FMD vaccine including serotypes O Pan Asia2, A Iran O5 and SAT2/EGY/2012 were prepared as formula 1- (50% carbomer to 50% antigen); formula 2 (50% Montanide ISA 206 to 50%antigen) and formula 3- (25% Montanide ISA 206 and 25% carbomer with 50 % antigen). All of such formulae were found to be free from foreign contaminants, safe and potent, showing no postvaccinal reactions and high protective levels of specific FMD antibodies in Guinea pigs. Each vaccine formula's immunogenicity was determined by estimation of 50% Guinea pig protective dose (GPPD50) and monitoring of the humeral antibody response of vaccinated G. Pig groups. It was found that Montanide oils 206 with carbomer is the best vaccine formula, followed by Montanide oils 206 and finally carbomer which give early short-lasting immunity.
Authors and Affiliations
Walaa S. Shabana, Abeer Abd EL-Sadek
Keywords
Related Articles
- EP ID EP693785
- DOI https://dx.doi.org/10.21608/javs.2021.154578
- Views 126
- Downloads 0
Effect of Applying Clove and Cinnamon Essential Oils to Milk Rice Pudding in Controlling Bacillus cereus and Bacillus subtilis Growth with Respect to the Sensory Traits
Milk rice pudding (MRP) is a commercial and popular dairy dessert, but owing to its characteristics and valuable ingredients, it may be contaminated by many pathogenic and spoilage microorganisms. So, this study aimed to...
Tracking Appearance and Development of the Ossification Centers in Cranium and Skull Base Bones of Iraqi Sheep Fetuses (Ovis aries) Using Double Stain Method
The skull is a group of flat and irregular bones that protect the brain and special sense organs. The shape of the head depends on the shape of the skull, which is closely related to certain structural and phenotypic fea...
Overview of tuberculosis in Animals in Nigeria
Tuberculosis of animal origin is currently in the increase, especially in the developing countries like Nigeria where animals and man share microenvironment together coupled with lack of proper control measures. The dise...
A Typical Case of Chronic Myeloid Leukemia (CML) in A Native Shepherd Dog in Duhok Province, Iraq
A typical case of a 7-years-old Kurdish male shepherd dog was referred with two weeks history of lethargy, restlessness, anorexia and severe emaciation. Physical examination of the dog revealed generalized lymphadenopath...
Cysteine enhance Holstein bullâs sperm assessment quality and conception rate when added to duck egg yolk low-density lipoproteins
Spermatozoa undergo a significant transformation during the cryopreservation process, including oxidative stressors during dilution, cooling, equilibration, or freezing and thawing stages that postpone their motility and...