Effect of cholesterol exposure on the expression of metallothionein in human retinal pigment epithelial cells
Journal Title: Eye Science (Yanke Xuebao) - Year 2021, Vol 36, Issue 2
Abstract
[Objective:] To study the effects of cholesterol, the main component of drusen, on the expression of metallothionein of ARPE-19 cells. [Methods:] The ARPE-19 cell line was cultured in vitro, and the cells were divided into a control group and a cholesterol treatment group (2.5 mg/mL). The treatment time was 0, 6, 12, 24, 48, 72 hours. Real-time quantitative PCR was used to detect the expression of hMT1a, hMT2a and hMT3 at the mRNA level, and Western blot was used to detect the expression at the protein level. [Results:] The mRNA expression of hMT1a, hMT2a and hMT3 were up-regulated by cholesterol and the protein expression of total MTs was increased with cholesterol treatment. [Conclusion:] Cholesterol, the main component of drusen, can up-regulate the expression of metallothionein in human retinal pigment epithelial cells, suggesting that the expression of metallothionine can be stimulated by the initial stage of drusen formation. However, whether its detection can be used for the early detection and early diagnosis of age-related macular degeneration or not still needs to be further explored.
Authors and Affiliations
Min ZHANG, Peng ZHANG, Hongli ZHAO
Keywords
Related Articles
- EP ID EP763475
- DOI 10.3978/j.issn.1000-4432.2020.12.07
- Views 10
- Downloads 0
Evaluation of the accuracy of subjective refraction according the negative relative accommodation and accommodation response
[Objective:] To observe the consistency of the objective results of primary small-pupil optometry and ciliary muscle paralysis in children with suspected myopia aged 8–15 years with different adjustment states, and to an...
Early capsular block syndrome after cataract surgery: A case report
Capsular block syndrome (CBS), a fairly rare complication of phacoemulsification, can develop into one of the three following possible clinical scenarios: intraoperative, early postoperative, and late postoperative, depe...
Perioperative experience in small incision lenticule extraction patients: a qualitative study
[Objective:] To investigate the perioperative experience after small incision lenticule extraction (SMILE). [Methods:] A semi-structured interview was conducted in 15 patients who underwent SMILE surgery in Zhuhai People...
Research progress in epiretinal macrophage-like cells characterized by en face OCT in ocular fundus diseases
Macrophage-like cells (MLC) refer to immune cells that originate from and function similarly to macrophages, including microglia, hyalocytes, and macrophages themselves. By setting the display level of en face OCT to the...
Influence factor of self-management among glaucoma patients undergoing day surgery
[Objective:] To determine self-management and its association with self-efficacy and knowledge among glaucoma patients undergoing day surgery. [Methods:] A total of 223 glaucoma patients were recruited from September 202...