Effect of Different Culture Media and Plant Growth Regulators on Callus Induction of Stevia Rebaudiana
Journal Title: International Journal of Farming and Allied Sciences - Year 2014, Vol 3, Issue 7
Abstract
The main objectives of this present study were to develop the optimal concentratioand combination of auxin and cytokinin for optimized callus induction through seeds implanting in MS medium. stevia is cultivated by seeds or stem cutting, but Seed germination of stevia is very poor. In this investigation, seeds of stevia were evaluated for in vitro callusing and demonstrated that callus induction in medicinal plant Stevia rebuadiana was affected by seed explants and different level of plant growth regulators. Callus induction was initiated trough seeds on MS media having auxins & cytokinins and responses were varied according to the plant growth regulators treatment. Whereas the application of IBA+BAP (3 or 3.5+2.5 mg/l) and NAA+IBA+BAP+KIN (0.5+3+1.5+1 mg/l) indicated most satisfactory performance on callus initiation and induction but the highest callusing (85%) was achieved on MS medium enriched with NAA+IAA+BAP (1.5+2.5+1.5 mg/l). Data showed positive correlation between presence of BAP and results proved that media with IBA+BAP (3.5+2.5mg/l) and NAA+IAA+BAP (1.5+2.5+1.5 mg/l) were better for callus induction of stevia respectively as compared to others.
Authors and Affiliations
K. Keighobadi ,M. Golabadi and F. Mortazeenezhad
Keywords
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