Effect of Different Doses of Sitagliptin in Treatment of Experimentally Induced Colitis in Mice
Journal Title: Journal of Pharmaceutical Research International - Year 2015, Vol 7, Issue 2
Abstract
Ulcerative colitis is a chronic, relapsing and progressive inflammatory bowel disease (IBD) characterized by diffused mucosal inflammation of the colon. DPP-IV inhibitors may provide a new treatment strategy for IBD. Thus, the aim of this study is to evaluate effect of different doses of sitagliptin as an early treatment of experimentally induced ulcerative colitis in mice. Sitagliptin was administered after the appearance of signs and symptoms of the disease as an early treatment. Twenty four mice were divided into four groups; control group, non treated DSS-induced colitis group, sitagliptin (20 mg/kg/d)-treated DSS-induced colitis group and sitagliptin (100 mg/kg/d)-treated DSS-induced colitis group. The disease activity index (DAI) was calculated by summarizing the scores for weight loss, stool consistency, hemoccult positivity. The length of colon was measured as an indirect marker of inflammation (rate of colon shortening) for each mouse. Serum tumour necrosis factor- α (TNF-α) was measured. Reduced glutathione, Nitrite, Malondialdehyde were measured in Colonic homogenates. Histopathological examination was done and the lesion was scored. Reduced glutathione in colonic homogenates was increased. Histopathological score was improved. It can be concluded that sitagliptin is partially effective for treatment of mice with experimentally induced ulcerative colitis.
Authors and Affiliations
Rania Elkatary, Karawan Abdelrahman, Amal Hassanin, Ahlam I. Elmasry, Amro El Karef, Hussein Abdalaziz Abdalla
Keywords
Related Articles
- EP ID EP344354
- DOI 10.9734/BJPR/2015/18241
- Views 100
- Downloads 0
Antidiabetic Activity of Different Fractions of Ethanolic Extract of Dactyloctenium aegyptium in Streptozotocin Induced Diabetic Rats
Aims: The present study was aimed to evaluate the antidiabetic effect of n-hexane, Chloroform, Ethyl acetate and Methanolic fractions from ethanolic extract of Dactyloctenium aegyptium in streptozotocin induced diabetic...
Adherence to Anti-retroviral Therapy among HIV-Positive Individuals in Ghana: The Role of Stress and Post Traumatic Stress Symptoms
Aims: To identify stress and post traumatic stress symptoms (PTSS) in HIV patients on Antiretroviral therapy (ART) and further assess the possible relationship between stress, PTSS, immune function and ART medication adh...
Biological Activities of Nitro Steroids
The present review describes the biological activities of synthetic nitro steroids. More than fifty biologically active nitro steroids showed an analeptic, antineoplastic, anesthetic, neuroprotector, immunosuppressant, d...
Tendency of Self-Medication among Various Malaysian Ethnicities
Aims: Self-medication has been observed in all kinds of societies regardless of region, religion, ethnicity and socioeconomic status. It is practiced by individuals as part of self-care for preventing or curing diseases....
Ameliorative Effects of Alcohol on Human Diabetic Volunteers – A Prospective Study
Aims: The purpose of this study is to assess and confirm the ameliorative effects of alcohol consumption on biochemical indices of blood i.e., blood glucose, HbA1c, NO2, NO3, lipid profiles, hs-CRP (high sensitive C–Reac...