Effect of increasing graded doses of formaldehyde on human embryonic kidney cells

Journal Title: Progress in Health Sciences - Year 2018, Vol 8, Issue 1

Abstract

Purpose: In the present study, we aimed to identify the effect of three increasing doses of most widely preservative, formaldehyde (FA) on the urinary system using human embryonic kidney cells (HEK-293) in vitro. Materials and methods: The HEK-293 cells were grown in Dulbecco’s Modified Eagle medium (DMEM, Gibco, USA) supplemented with 10% of fetal bovine serum, 100 IU/ml penicillin and 100 μg/ml of streptomycin (Gibco, USA) using 25cm2 flasks (Nunc, Denmark), in a CO2 incubator (Heal Force) at 37°C are treated with 19 % formaldehyde in DMEM supplemented with 10% of fetal bovine serum to yield final concentrations of 0.05 μl/ml, 0.1 μl/ml, 0.5 μl/ml and 1.0 μl/mlof 19% FA. Results: The result showed that statistically significant dose dependent decrease in cell viability of HEK-293 cells with exposure to increasing concentration of FA (0.05 μl/ml, 0.1 μl/ml, 0.5 μl/ml and 1.0 μl/ml) on MTT assay. Extremely huge dose dependent DNA damage with a dose dependent up regulation of mRNA expression of pro-apoptotic iNOS and TNF- gene was observed when HEK-293 cells are treated with the increasing concentration of FA. In addition, the protein expression levels of Bcl-2 and Bax revealed that increasing concentration of FA on HEK-293 cells down regulated the anti-apoptotic Bcl-2 and up regulated pro-apoptotic Bax gene. Conclusions: Our result indicates that, high dose of FA is more dangerous than the low dose on HEK-293 cells with cellular damage.<br/><br/>

Authors and Affiliations

A. Banerjee, M. Dholey, S. Mukherjee, B. K. Maji

Keywords

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  • EP ID EP346736
  • DOI -
  • Views 72
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How To Cite

A. Banerjee, M. Dholey, S. Mukherjee, B. K. Maji (2018). Effect of increasing graded doses of formaldehyde on human embryonic kidney cells. Progress in Health Sciences, 8(1), 36-45. https://europub.co.uk./articles/-A-346736