Effect of Invitro Zinc (II) supplementation on Normal and Cancer Cell lines
Journal Title: International Journal for Research in Applied Science and Engineering Technology (IJRASET) - Year 2015, Vol 3, Issue 1
Abstract
Zinc is the most abundant trace element which has role in genetic stability and function, present in the cell nucleus, nucleolus and chromosomes, and stabilizes the structure of DNA, RNA and ribosomes and found in many Zinc binding proteins. The purpose of this study is to access the Zinc cytotoxicity, total cellular Zinc content and total Zinquin Acid (Fluorophore) interaction with cellular Zinc in response to six different Zinc gradient medium using MTT assay, Atomic absorption spectroscopy and Fluorescence spectroscopy respectively. Statistical analysis one way ANOVA and Tukey HSD test for mean comparison was performed. Our findings are, significant differences on normal cell line survival of 108.7% at 10 µM supplemented medium was seen. Cytotoxicity was seen at 80 µM of 89.78% and 86.46% in MDA-MB-231 and U-87-MG Cell lines respectively. Significant increase in cellular influx and accumulation of Zn2+ ions was observed in all cell lines. Fluorescence intensity emission peak response increased with supplementation of gradient medium, which supported the hypothesis of increased cellular Zinc accumulation, λ-max shifting to shorter wavelength i.e. idea supports blue shift emission of Zinquin Acid which interacts not only with free labile Zinc ions but with Zinc bound proteins. Zinc based fluorescence probes help detection of altered expression of Zinc.
Authors and Affiliations
Sandeep Adhikari, Basanta Lamichhane, Pritish Shrestha, Bhupal Govinda Shrestha
Keywords
Related Articles
- EP ID EP19344
- DOI -
- Views 311
- Downloads 4
Estimating of long term concrete magnesium sulfate resistance up to 450 days depending on cement impact and water cement ratio
Sulfate attack is a complex form of deterioration that has damaged concrete structures throughout the world. Sulfate attack is particularly complex because the source of sulfates can be external or internal. The sulfate...
A Survey on Web Mining: Overview, Techniques, Tools, and Applications
Web Mining is moving the World Wide Web towards a more useful environment in which users can quickly and easily find the information they need. It uses document content, hyperlink structure, and usage statistics to assi...
Finite Element Thermal Analysis of Ceramic Coated Aluminium Silicon Alloy on Piston
the last few years the usage is increase in the utilisation of aluminium-silicon alloys, especially in the automobile industries, due to their high strength to weight ratio, wear resistance, low density, low coefficient...
A Case Study on Different Lighting Systems
Techno-economic performance comparison of Compact Fluorescent Lamps (CFL) with Light Emitting Diodes (LED), fluorescent tubes, incandescent bulbs was carried out in view of worsening power and energy crisis. However, tu...
Image Compression & Decompression using Discrete Wavelet Transform Approach
Image compression deals with reducing the amount of data required to represent a digital image. When multimedia data such as images, video and audio is not compressed then these data requires vast amount of computer sto...