Effect of L- Carnitine and COQ10 Addition to SMARTProMedium on Human SpermConcentration,SpermMorphology and Chromatin StructureduringIn VitroSperm Activation
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2017, Vol 12, Issue 2
Abstract
This study was designed to assess the effect of different concentrations of L-carnitine and Co enzyme Q10 (COQ10) supplied to SMART Pro- medium on human sperm concentration, sperm morphology and chromatin structure during in vitro sperm activation. Eighty seven samples of semen of infertile and fertile men were randomly collected at the High Institute for Infertility Diagnosis and Assisted Reproductive Technologies/Al- Nahrain University. The mean age of the men was (32.034 ± 0.57) years with mean duration of infertility (4.644 ± 0.25) years. Seminal fluid analyses were done involving macroscopic and microscopic examinations according to WHO criteria. Swim-up technique was dependent for in vitro sperm activation; the washed samples were divided before using centrifugation swim-up technique into 3 groups: control group (G1) without L-carnitine and CoQ10. While, in G2 and G3 two concentrations of L-carnitine (20µg, 40µg) and CoQ10 (20µg, 40µg) were used respectively. Moreover, all groups within post-activation enriched with SMART- Pro media. The sperm concentration, sperm morphology and chromatin structure were evaluated after the addition of L-carnitine and Co Q10. The results showed a Significant (P<0.05) differences in sperm parameters of post-activation when compared to the pre-activation. The results for all semen samples of post activation revealed a significant (P< 0.05) decrease in sperm concentration while, a significant (P<0.05) increase in the percentage of normal sperm morphology as compared to pre- activation. The percentage of normal sperm morphology in 40µg (G3) group showed significant (P<0.05) increase as compared to control group (G1) with no significant (P>0.05) differences with 20µg (G2) group. However, 40µg (G3) group provides improvement in the percentages of normal sperm morphology as compared with 20µg (G2) group with no significant (P>0.05) between G2 and G3. A significant (P<0.05) decrease in fragmented sperm DNA percentage of post activation was seen when compared with the pre-activation with non-significant (P>0.05) differences between G2 and G3. Moreover, DNA fragmentation in the spermatozoa treated by 40µg L-carnitine and CoQ10 was the lowest among post-activation groups. From the results of the study, it can be concluded that the addition of 40 µg of L-carnitine and CoQ10 to washed sperms can improve sperm concentration and normal sperm morphology in vitro.
Authors and Affiliations
Majida H. Mutlag Al-Shammari1, Jabbar H. Yenzeel, Muhammad-Baqir M-R Fakhrildin2
Keywords
Related Articles
- EP ID EP388817
- DOI 10.9790/3008-1202045155
- Views 65
- Downloads 0
Evaluation of some Serum cytokine and adipokines profile in Iraqi celiac patients before and after treatment
Celiac disease (CD) is main gastrointestinal disease initiated by an immune reaction to gluten in genetically susceptible people. The objective of this analysis attempted to evaluate the nature as well as effect of syste...
Betterment the production of prodigiosin that was produced from Serratia marcescen by means mutations
Serratia marcescens was exposed to mutation for becoming better the production of prodigiosin (quantitatively and qualitatively) and examined it as antibacterial activities. One isolate of S.marcescen (Wild) was exposed...
Antioxidant Production Of Lactic Acid Bacteria Isolated From Indonesian Traditional Fermented Buffalo Milk (Dadih)
One of antioxidant sources is foods that contain peptides from enzymatic hydrolysis and/or fermentation by lactic acid bacteria (LAB). The availability of antioxidants in fermented food, particularly milk, is associated...
Colon Targeting of Naringin for Cytoprotection against Ulcerative Colitis: In Vitro-In Vivo Study
Naringin has low oral bioavailability because of its degradation at the upper gut. Therefore, this study involved formulation of compression-coated tablets of naringin using mixtures of Eudragit L100-55 (EUD-L) and hydro...
Physicochemical Properties and in Vitro Anti-Inflammatory Effects of Indian Chia (Salvia Hispanica L.) Seed Oil
Salvia hispanica L. also known as chia or salba has been used as a fodder/food in Central America and was one of the chief dietary components of the ancient culture. Owing to the fatty acid profile and nutrition value ch...