Effect of L-carnitine on platelet bacterial contamination and platelet metabolism during 5 days of storage of platelet concentrates
Journal Title: Khoon - Year 2018, Vol 15, Issue 1
Abstract
Effect of L-carnitine on platelet bacterial contamination and platelet metabolism during 5 days of storage of platelet concentrates Velashjerdi Z.1, Deyhim M.R.2, Razjou F.2, Eydi A.1 1Science and Research Branch, Islamic Azad University, Tehran, Iran 2Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran Abstract Background and Objectives Platelet concentrate is one of the important blood products. The platelets are stored at 22±2°C that is an optimum temperature for bacterial growth, so the platelet bacterial contamination is considered to be the most common infective risk factor in blood transfusion. Materials and Methods In this experimental study, we selected ten platelet concentrates from healthy donors prepared by Iranian Blood Transfusion Inovation Center and stored at 22º C with gentle agitation up to 5 days. Then, the antibacterial effects of LC on the platelet concentrates inoculated with the gram-positive Staphylococcus epidermidis as well as the effects of LC on the metabolic parameters of the platelets were measured. Results The results showed that L-carnitine in concentration of 50 mM could significantly decrease the growth of 1.5×106CFU/ml Staphylococcus epidermidis (p < 0.01). Moreover, the concentration of 50 mM could better maintain the LDH enzyme activity in the platelet concentrate during storage (P day3 = 0.003, P day5 = 0.002). Lactate concentration in LC treated platelet was lower than the control group at day 5 of storage (P day5 = 0.008) and also pH of the platelet was better maintained in LC treated platelets. In addition, the platelet count showed less decrease in LC treated platelets compared with control group at day 5 of storage (P day5 = 0.007). Conclusions L-carnitine can improve the metabolism and quality of the platelet concentrates, as an additive, during the storage and it can decrease bacterial growth in the platelet concentrates during the storage. Key words: L-Carnitine, Blood Platelets, Metabolism Received: 20 Sep 2017 Accepted:17 Jan 2018 Correspondence: Deyhim MR., PhD of Clinical Biochemistry. Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine. P.O.Box: 14665-1157, Tehran, Iran. Tel: (+9821) 88601555; Fax: (+9821) 88601555 E-mail: mrdeyhim2017@gmail.com
Authors and Affiliations
Z. Velashjerdi, M. R. Deyhim, F. Razjou, A. Eydi
Keywords
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