Effect of low-frequency repetitive transcranial magnetic stimulation on autism-like behavior in BTBR T+ Itpr3tf/J mice
Journal Title: Chinese Journal of Nervous and Mental Diseases - Year 2024, Vol 50, Issue 2
Abstract
[Objective] To investigate the effect of low-frequency repetitive transcranial magnetic stimulation (rTMS) on the BTBR mice, one of the idiopathic autism spectrum disorder mouse models. [Method] Male BTBR and B6 mice were randomly assigned to the BTBR+1 Hz group, BTBR group, B6+1 Hz group, and B6 group. At six weeks of age, 1 Hz rTMS treatment was administered to the BTBR+1 Hz and B6+1 Hz groups and false stimulation treatment was administered to the BTBR and B6 groups at the same time. Behavioral tests were conducted to test sociability, repetitive behaviors, locomotion, anxiety, and depression, after which IL-1β, IL-6, IL-18, IL-23, TNF-α, and IFN-γ in the cortex and hippocampus were measured. [Result] The mice in the BTBR group had a shorter social duration. Compared to the mice in the B6 group, there were significant differences in self-grooming duration, the levels of IL-1β, IL-18, and TNF-α in the cortex and IL-1β, IL-6, IL-18, TNF-α, and IFN-γ in the hippocampus of the mice in the BTBR group (P<0.05). After treatment with rTMS, the three-chambered social approach task showed that the mice in the BTBR+1 Hz group had a longer time in sociability recognition [(237.60±40.80) s vs. (184.10±23.94) s, P=0.019] and social novelty recognition stage [(196.80±61.17) s vs. (260.50±48.59) s, P=0.013]. In the self-grooming test, the mice in the BTBR+1 Hz group had a shorter grooming time than the mice in the BTBR group [(33.35±7.74) s vs. (81.95±38.71) s, P=0.001]. The levels of IL-1β, IL-18 and TNF-α (P<0.05) in the cortex and IL-1β, IL-6, IL-18, TNF-α and IFN-γ (P<0.05) in the hippocampus of the mice in the BTBR+1 Hz group were significantly reduced compared with the mice in the BTBR group. In addition, the BTBR mice buried more marbles than the B6 mice (P<0.01), and 1 Hz rTMS could significantly reduce the level of IL-23 and IFN-γ in the cortex (P<0.05) and the level of IL-23 in the hippocampus (P<0.05). [Conclusion] 1 Hz rTMS improves social deficits and reduces repetitive behavior in BTBR mice. These effects may be associated with the reduction in elevated neuroinflammatory response in the cortex and hippocampus.
Authors and Affiliations
Xinyi WANG, Yulong CAI, Yanna LI, Linying YUAN, Yanfan HUA, Yunzi LI, Wenya LAN, Xinfeng LIU
Keywords
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- EP ID EP750422
- DOI 10.3969/j.issn.1002-0152.2024.02.004
- Views 63
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