Effect of Orthosiphon stamineus Extract on HIF-1Α, Endothelin-1, and VEGFR-2 Gene Expression in NRK-52E Renal Tubular Cells Subjected to Glucotixicity
Journal Title: International Journal of Experimental Research and Review - Year 2024, Vol 37, Issue 1
Abstract
This study aimed to investigate the impact of Orthosiphon stamineus extract on gene expression in NRK-52E cells under conditions of glucotoxicity. Gene expression analysis using RT-PCR was conducted following exposure of cultured NRK-52E cells to glucotoxic conditions and various concentrations of Orthosiphon stamineus extract. The results revealed a dose-dependent decrease in HIF-1α, ET-1 and VEGFR-2 gene expression levels upon treatment with Orthosiphon stamineus extract. The diverse array of phytochemicals found within Orthosiphon demonstrates a significant impact on the biochemical pathways implicated in the pathogenesis of the disease. Additionally, molecular docking studies suggested the potential inhibition of Rho-kinase as a mechanistic explanation for this observed effect. These findings suggest that Orthosiphon stamineus extract may possess renoprotective properties against glucotoxicity-induced cellular damage. This study contributes to understanding potential therapeutic interventions for managing renal complications associated with conditions such as diabetes.
Authors and Affiliations
Devaprasad Markandeyan, Shiek S. S. J. Ahmed, Palani Perumal, Arulvasu Chinnasamy, Bhavatarini Govindaraj, Niranjni Sekar, Sudhan Mookandi, Priyadarshini Shanmugam, Janakiraman Velayudam, Deepak Rajasekar Padmanaban, Perumal Jayaraman
Keywords
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- EP ID EP733359
- DOI 10.52756/ijerr.2024.v37spl.015
- Views 45
- Downloads 1
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