Effect of senescence marker protein 30 on myocardial injury after myocardial infarction in mice through NLRP3 / Caspase1 inflammatory signaling pathway
Journal Title: Journal of Air Force Medical University - Year 2023, Vol 44, Issue 8
Abstract
Objective To investigate the effect of SMP30-/ - on myocardial inflammatory signaling pathways and myocardial injury after acute myocardial infarction (AMI). Methods Thirty wild-type ( WT) C57BL / 6J mice and thirty SMP30-/ - mice were randomly divided into WT sham operation group (WT + Sham group), WT myocardial infarction group (WT + MI group), SMP30-/ - sham operation group ( SMP30-/ - + Sham group), SMP30-/ - myocardial infarction group ( SMP30-/ - + MI group) . The MI groups were treated with ligation of the left anterior descending coronary artery for modeling, while the Sham groups were treated with no ligation. After 28 d of modeling, the small animals were detected by ultrasonography and sacrificed after isoflurane anesthesia for sampling. HE and Masson staining were performed respectively, the content of reactive oxygen species related to oxidative stress was detected by ELISA, and the expression levels of oxidative stress and pyroptosis-related proteins were detected by Western blotting. Results Compared with the WT + Sham group, the expression of SMP30 significantly decreased in the WT + MI group ( P < 0. 05). Compared with the WT + MI group, SMP30-/ - significantly aggravated the cardiac function injury and the degree of myocardial fibrosis in the myocardial infarction area (P < 0. 05, P < 0. 01) in the SMP30-/ - + MI group. Further tests revealed that SMP30-/ - significantly increased myocardial oxidative stress and inflammatory damage in the SMP30-/ - + MI group (P < 0. 05). Conclusion The expression of SMP30 is significantly down-regulated in AMI tissues. SMP30-/ - can aggravate the cardiac function injury in mice with myocardial infarction, enhance the activation of NLRP3 / Caspase1 inflammatory pathway, and lead to further ventricular remodeling.
Authors and Affiliations
JIANG Liqing, ZHANG Bin, ZHU Hanzhao, ZHANG Liyun, JIN Zhenxiao, YU Shiqiang, DUAN Weixun
Keywords
Related Articles
- EP ID EP724917
- DOI -
- Views 46
- Downloads 0
Analysis of risk factors for recurrent adenomatous polyps and colorectal cancer one year after resection of high-risk adenomas
Objective To investigate the risk factors for recurrent adenomatous polyps and colorectal cancer (CRC) one year after resection of high-risk adenomas. Methods A retrospective analysis was conducted on 683 patients who...
Investigation and evaluation of dietary nutrition of cadets in an Air Force college during field training
Objective To understand the dietary intake of cadets in an Air Force college during field training and carry out nutritional analysis, so as to provide a reference for the military dietary nutrition support. Methods Die...
Breast reconstruction with prepectoral prosthesis implantation: a better one-stage reconstruction for mammectomy
Breast cancer is the malignant tumor with the highest incidence in women, and the treatment of early breast cancer is surgery. In order to reduce the psychological burden of patients and improve the function of upper li...
Comparison of clinical efficacy of all-ceramic inlay and composite resin inlay in restoration of posterior Class Ⅱ cavity
Objective To study the repair effects of all-ceramic inlay and composite resin inlay and the changes in the levels of matrix metalloproteinase-3 (MMP-3) in gingival crevicular fluid after restoration, and to evaluate th...
Effect of extracellular signal-regulated kinase on condylar cartilage degeneration of temporomandibular joint osteoarthritis
Objective To explore the effect of extracellular signal-regulated kinase ( ERK) on condylar cartilage degeneration of temporomandibular joint osteoarthritis (TMJOA) in mice. Methods Thirty-six female C57BL / 6J mice wer...