EFFECT OF STEM CELL FACTOR ON CAPRINE PREANTRAL FOLLICLE CULTURE
Journal Title: International Journal of Agriculture Sciences - Year 2016, Vol 8, Issue 50
Abstract
The influence of stem cell factor on in-vitro culture of preantral follicles (PFs) isolated from goat ovaries was investigated. Preantral follicles were isolated from the cortex of goat ovaries and individually cultured for 10 days in different concentrations (25 ng/ml, 50 ng/ml, 100 ng/ml) of stem cell factor (SCF).The proportion of PFs exhibiting growth, increase in diameter and antrum formation was assessed to determine the in vitro development. The oocytes isolated from cultured PFs were kept in-vitro maturation medium for 27 h, to assess the nuclear maturation to the metaphase II (M II) stage. The proportion of PFs exhibiting growth, average increase in diameter, antrum formation, extrusion of oocytes and meiotic resumption of oocytes were highest when the culture medium was supplemented with 50 ng/ml of SCF individually. Based on the best concentration from the above results, the preantral follicles were cultured with thyroxin (T4), follicle stimulating hormone (FSH), stem cell factor (SCF) and leptin in various combinations. It is concluded that in vitro culture of goat PFs in combination of stem cell factor and leptin i.e SCF + leptin (31.25 ± 1.15) did not improve the frequency of oocyte maturation to metaphase II stage when compared with SCF alone.
Authors and Affiliations
V. DIVYA
Keywords
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