Effect of synthetic biologically activated 45S5 glass nanoparticles on osteogenesis differentiation of mesenchymal human bone marrow
Journal Title: Khoon - Year 2019, Vol 15, Issue 4
Abstract
Effect of synthetic biologically activated 45S5 glass nanoparticles on osteogenesis differentiation of mesenchymal human bone marrow Shams M.1, Salimi A.2, Ghollasi M.3, Halabian R.4 1Institute of Materials and Energy(MERC), Karaj, Iran 2Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran 3Faculty of Biological Sciences, Kharazmi University, Tehran, Iran 4Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran Abstract Background and Objectives Glasses and glass-ceramics are a group of biological substances that form hydroxyapatite against a simulated solution of the body and can be used in many clinical cases that require the production and repair of bone. The purpose of this study was to create a 45S5 bioactive glass nanoparticle and evaluate its impact on staining and differentiation of mesenchymal stem cells into bone cells. Materials and Methods In this experimental research, the nanocomposite of the bioaccuminant 45S5 was synthesized by the fusion method and planetary mill was converted to the nanoscale structure; then, its physicochemical and structural properties were investigated. The bioactivity was evaluated using a simulated body solution. The growth, amplification and differentiation of mesenchymal stem cells in the vicinity of nanoparticles were investigated. Results Biomedical evaluation indicated the formation of hydroxyapatite on nanoparticles after zinc immersion in the body was simulated. Cell experiments also confirmed the lack of toxicity of the glass nanoparticles and its stimulating effect for the growth, proliferation and differentiation of mesenchymal cells in bone cells. In the bone differentiation, the activity of alkaline phosphatase in the glass nanoparticle was expressed after 14 days of differentiation (0.55 ± 0.07), while it was the control sample (0.15 ± 0.03). Conclusions According to the progenies, mesenchymal stem cells can propagate and grow on the nanoparticles of the synthesized bioactive glass and, in addition to not being toxic, stimulate and stimulate cell growth. Key words: Nanoparticles, Mesenchymal Stem Cells, Glass Received: 4 Jul 2018 Accepted: 2 Sep 2018 Correspondence: Halabian R., PhD in Medical Biotechnology. Assistant professor of Faculty of Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sieances. P.O.Box: 14359-44711, Tehran, Iran. Tel: (+9821) 86072709 ; Fax: (+9821) 86072709 E-mail: r.halabian@yahoo.com
Authors and Affiliations
M. Shams, A. Salimi, R. Halabian
Keywords
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