Effect of temperature on storage of ethionamide during susceptibility testing
Journal Title: Journal of Microbiology and Infectious Diseases - Year 2013, Vol 3, Issue 3
Abstract
Objective: Ethionamide being thermolabile in nature, effect of temperature on the drug and its stability in liquid and solid media during susceptibility testing procedures was assessed to understand the inconsistency in DST formats. Methods: Working solution of ethionamide and Lowenstein- Jensen (LJ) media incorporated with ethionamide were preincubated at 4°C and 37°C prior to DST methods was incubated till 4 weeks at different temperatures and utilized for DST in MGIT 960. Results: Degradation of ethionamide working solution was observed at 37°C after 3 weeks of incubation. Ethionamide incorporated LJ media can be stored without compromise on susceptibility up to 5 weeks. But, a week of prior incubation at 37°C has deleterious effect on the DST profile. Ethionamide was found to degrade at 37°C after different time points when stored as solution or as LJ media. Conclusion: Use of DST formats that provide results within 2 weeks can be recommended for ethionamide susceptibility testing. J Microbiol Infect Dis 2013; 3(3): 128-132 Key words: Degradation; Ethionamide; LJ medium; MGIT 960; Mycobacterium tuberculosis
Authors and Affiliations
Rajagopalan Lakshmi, Ranjani Ramachandran, Syam Sundar A, Baskaran M, Anandan M, Thiyagarajan V, Vanaja Kumar
Keywords
Related Articles
- EP ID EP115183
- DOI 10.5799/ahinjs.02.2013.03.0095
- Views 121
- Downloads 0
İnsan barsağında gastrodüedonal hastalıklara yol açan Heliccobacter pylori’nin kalıcı kolonizasyonu
Helicobacter pylori midenin epitelyal hücre hattında kolonize olan bir barsak bakterisi olup burada uzun süre sebat eder. Dünya nüfusunun yaklaşık üçte ikisi H. pylori ile enfektedir ve ülserlerin % 90’ından fazlasının...
Campylobacter fetus subsp.fetus bacteremia in a patient with myelodysplastic syndrome: A case report
Campylobacter fetus is an important pathogen of extra intestinal infections in patients with immunodeficiency. A case of Campylobacter fetus subsp. fetus bacteremia in a patient with myelodysplastic syndrome was present...
Knowledge, Attitude and Pattern of Antibiotic Usage among Students of a Nigerian University
Objective: Antibiotic resistance is a major public health issue globally fueled largely by its misuse. Controlling this problem would require an understanding of the levels of awareness of the population towards antibiot...
Veziküler ve makülopapüler döküntü ve febril nötropeni ile başvuran bir bruselloz olgusu
Bruselloz, tüm doku ve organları etkileyebilen sistemik bir hastalıktır. Bruselloz farklı belirtiler ile ortaya çıkabilir ve semptomlar özgül değildir. Klinik bulgular geniş bir yelpazede görülebilir, bu yüzden tanısı zo...
Disseminated Nocardiosis in an Immunocompetent Adult: Diagnostic Challenges
Nocardiosis is an opportunistic infection seen in people with impaired host defenses usually cell mediated immunity. It can involve a single organ or present as disseminated infection affecting multiple organ systems. We...