Effect of temperature on storage of ethionamide during susceptibility testing
Journal Title: Journal of Microbiology and Infectious Diseases - Year 2013, Vol 3, Issue 3
Abstract
Objective: Ethionamide being thermolabile in nature, effect of temperature on the drug and its stability in liquid and solid media during susceptibility testing procedures was assessed to understand the inconsistency in DST formats. Methods: Working solution of ethionamide and Lowenstein- Jensen (LJ) media incorporated with ethionamide were preincubated at 4°C and 37°C prior to DST methods was incubated till 4 weeks at different temperatures and utilized for DST in MGIT 960. Results: Degradation of ethionamide working solution was observed at 37°C after 3 weeks of incubation. Ethionamide incorporated LJ media can be stored without compromise on susceptibility up to 5 weeks. But, a week of prior incubation at 37°C has deleterious effect on the DST profile. Ethionamide was found to degrade at 37°C after different time points when stored as solution or as LJ media. Conclusion: Use of DST formats that provide results within 2 weeks can be recommended for ethionamide susceptibility testing. J Microbiol Infect Dis 2013; 3(3): 128-132 Key words: Degradation; Ethionamide; LJ medium; MGIT 960; Mycobacterium tuberculosis
Authors and Affiliations
Rajagopalan Lakshmi, Ranjani Ramachandran, Syam Sundar A, Baskaran M, Anandan M, Thiyagarajan V, Vanaja Kumar
Keywords
Related Articles
- EP ID EP115183
- DOI 10.5799/ahinjs.02.2013.03.0095
- Views 118
- Downloads 0
Fosfomisinin klinik izolatlara karşı in vitro etkinliği
Amaç: Fosfomisin komplike olmayan üriner sistem enfeksiyonlarının tedavisinde alternative bir ilaçtır. Bu çalışmanın amacı fosfomisinin in vitro etkinliğini, metisiline dirençli Staphylococcus aureus (MRSA), metisiline d...
Persistent colonization of Helicobacter pylori in human gut induces gastroduodenal diseases
Helicobacter pylori are gut bacteria colonize in the epithelial cell lining of the stomach and persist there for long duration. Around two-thirds of the world’s populations are infected with H. pylori and cause more th...
Boosted- lopinavir versus boosted- atazanavir plus two nucleoside reverse transcriptase inhibitors in second- line antiretroviral therapy in HIV-1 infected patients in Abidjan, Ivory Coast
Introduction: Atazanavir is a protease inhibitor recently introduce in the therapeutic arsenal for second-line antiretroviral therapy in Ivory Coast. The objective of this study was to compare the efficacy and safety of...
Identification of Ochrobactrum oryzae in Primary loodstream nfection in a ialysis Patient: Can it be an Emerging Pathogen
Ochrobactrum spp is a gram-negative bacillus currently considered an emerging and opportunistic infection, rare in humans, and generally associated with indwelling foreign bodies. We report a case of primary bloodstream...
Correlation between Serum Calcium Levels and Smear Grading Among the Pulmonary Tuberculosis Patients
Objective: To find the serum calcium (Ca) levels in the pulmonary tuberculosis (PT) patients and its correlation with smear grading. Methods: Microscopically confirmed PT patients were included in the test group and heal...