Effect of the EGTA (Ethylene Glycol Tetraacetic Acid) Supplementation in the Freezing Extender on Quality of Cryopreserved Bull Sperm
Journal Title: International Journal of Veterinary Science and Research - Year 2017, Vol 3, Issue 1
Abstract
The aim of this study was to evaluate the effect of the inclusion of divalent ion chelating agent EGTA (ethylene glycol tetraacetic acid) in the freezing extender on quality of cryopreserved bull sperm. The freezing extender (egg yolk Tris glycerol) was supplemented with 0 (non-supplemented), 1, 5, 10 and 15 mM EGTA. Bull sperm EGTA improved post thaw sperm quality but in different parameters according to the concentration of EGTA, 5 mM improved the acrosomal state and 1 mM the motility. Our results suggest the EGTA supplementation in the freezing extender of bull sperm might improve the post-thawing sperm quality.
Authors and Affiliations
Posado R, García JJ, Gómez-Fernández J, Tomás C, Gómez-Izquierdo E, de Mercado E
Keywords
Related Articles
- EP ID EP554120
- DOI 10.17352/ijvsr.000017
- Views 50
- Downloads 0
Functional Properties (Acid and Bile Tolerance) and Antibiotic Susceptibility of Lactic Acid Bacteria Isolated from Newborn Calves for the Design of a Probiotic Product
Diarrhea in young calves in dairy farms is one of the main causes of economic losses, morbidity and mortality. The use of probiotic products as feed additives or complements is a novel alternative for the prevention of i...
Factors Affecting Lethality of Bisphenol a on Biomphalaria alexandrina Snails
The present study was undertaken to investigate the influence of some environmental factors including temperature, water vegetation, bed mud and pH on LC50, LC90and LT50 of bisphenol A (BPA) on the snail host of Schistos...
Renal Protective effects of Melatonin in rat treat by Gemcitabine
Nephrotoxic side effects of gemcitabine have been reported in patients receiving this drug medication. Melatonin is a potent free radical scavenger and antioxidant and has protective effects against ischemic damage. In...
Prevalence of Major Intestinal Nematodes of Equines in Jimma Town, South Western Ethiopia
A cross sectional study was conducted from October, 2015 up to March, 2016 in Jimma town, Oromia Regional State of South West Ethiopia, with the objective of determining the prevalence of intestinal nematode parasites of...
Factors affecting Somatic Cell Count in milk of dairy cows in Costa Rica
Somatic cells, such as bacteria fighting leukocytes, are present in cow’s milk as an immune response to combat intramammary infection. The primary objective of this study was to determine the effect of various factors on...