Effect of the extract from Gundelia tournefortii on immature mouse oocytes
Journal Title: Journal of Herbal Drugs - Year 2014, Vol 5, Issue 2
Abstract
Background & Aim: Considering that antioxidants are known as effective free radicals scavenger, it may be able to improve the in vitro oocyte maturation and the fetal quality. This study was designed to determine the effect of Gundelia tournefortii leaves (GTE) hydro-alcoholic extract as a source of antioxidant on in vitro oocyte maturation. Experimental: Germinal vasicle (GV) were recovered from 6-8 weeks old NMRI ovaries. GV were cultured for 24 hours in maturation medium in MEMα supplemented with 7.5 IU/ml hCG, 100 mIU/ml rhFSH, 5% FCS (control group) and adding different doses of GTE extract (group I: 10 µg/ml, group II: 20 µg/ml, and group III: 40 µg/ml) and then in vitro maturation stages and resumption of meiotic in all groups was recorded by inverted microscope. Results: In group I, maturation rates were improved compared to the control group. But this difference was not significant. In group II, maturation rates showed a significant increase compared to the control group (p<0.05). In group III, also maturation rates showed a significant increase compared to the control group (p<0.05). Recommended applications/industries: The results of this study showed that the extract from Gundelia tournefortii leaves has a positive effect on oocyte maturation that is doses dependent. With increasing concentration of the extract, the rate of maturation immature oocytes increased.
Authors and Affiliations
Azadeh Abedi; Leila Rouhi; Abdollah Ghasemi Pirbalouti
Keywords
Related Articles
- EP ID EP23448
- DOI -
- Views 338
- Downloads 21
Antibacterial activity of the essential oils of endemic plants
Background & Aim: Secondary metabolites with bioactive and functional properties in essence of aromatic plants can be useful for improvement of quality and persistence of food as natural preservative. The aim of this st...
Effect of Hydro-alcoholic Extract of Punica Granatum L. Peel on Ethylene Glycol-Induced Kidney Calculi in Wistar Rats
Background & Aim: This study evaluated prevention effect of hydro-alcoholic extract of punica granatum Peel ethylene glycol-induced Nephrolithiasis in wistar rats. Experimental: 20 male Wistar rats were randomly divided...
The essential oils of some medicinal plants on the immune system and growth of rainbow trout (Oncorhynchus mykiss)
Background & Aim: In present study, some of the Iranian endemic medicinal plants were used to evaluate potential as well as therapeutic agent and immunostimulants in aquaculture. The essential oils of five species from l...
Rapid screening of antioxidant activity, fracture rate and scavenging of free radicals by hairy root of Periwinkle (Catharanthus roseus L. G. Don)
Background & Aim: Oxidative stresses caused by free radicals are known to promote many diseases such as inflammation and cancer; therefore, any plant product that can inhibit free radical production can play an importa...
The effect of Policosanol on decrease of cholesterol, triglyceride and the other lipid metabolite levels in blood of Wistar rats
Background & Aim: Policosanol is a component from main alcohol groups with long-chain that extracted from cane sugar. In the present research, the effect of policosanol was investigated on decrease of lipid metabolite le...