Effect of vitamin B12 addition to extenders on semen in Awassi rams
Journal Title: Journal of Research in Ecology - Year 2018, Vol 6, Issue 1
Abstract
This study was aimed to investigate the effects of vitamin B12 and different cryopreservation techniques on some Awassi ram sperm parameters and sperm mitochondrial apoptosis. Semen samples were collected form five Awassi rams, evaluated and pooled. Fresh semen was diluted with Tris extender containing 0, 0.5, 1, 2, 4µg/ml and was cooled at 5°C and equilibrated for 2h. The obtained Semen was packed in 0.25ml cryovial. The results of this study showed that there were significant(P<0.05) increase of nitrogen vapour for a period of 10 min in the study characteristics of semen in comparison with 5min of the samples exposed to nitrogen vapour. The addition of low concentrations of vitamin B12 enhances the studied characteristics in comparison with the optimal and high concentrations of vitamin B12 (0, 2, 4µg). There seen a significant effect of interaction between the exposed samples to nitrogen vapour for a period of 10 min and at 0.5 and 1µg concentration of B12 in the studied characteristics. Use of 0.5 and 1µg of vitamin B12 with nitrogen vapour technique (10min) improves the sperm parameters post- thawing
Authors and Affiliations
Ahmed Younis Saieed, Khalaf AH Al-Rishdy, Zainab A Mahadi, Hobi A Abd-Alkareem
Keywords
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