Effectiveness and efficiency of mutagen on leaf chlorophyll in Hyacinth bean Lablab purpureus (L.) Sweet
Journal Title: International Journal of Life Science - Year 2018, Vol 6, Issue 1
Abstract
The seeds of Hyacinth bean (Lablab purpureus L.) Sweet varieties Konkan bhushan and Konkan Wal-2 were treated with various doses of chemical (EMS- 0.05%, 0.10%, 0.15% and SA- 0.010%, 0.015%, 0.020%) and physical (Gamma rays- 5KR, 10KR, 15KR) mutagen and were sown in the field with control and harvested in bulk to raise M1 generation to observe the characters and number of mutants in each population. to observe the chlorophyll mutation and leaf morphology. Chlorophyll mutation frequency was calculated on the basis of plant population. A distinct range of chlorophyll and morphological mutants were observed in the M1 generations. Four different types of spectrum of chlorophyll mutant’s viz. albina, xantha, viridis and chlorina were identified in the treated population. Various morphological aberrations had been observe viz. Bilobed leaf, reduction in size of leaves, cotton shape leaf, obtuse and retuse apex, notch at margin, sinuate leaf margin, lanceolate leaf, wrinkled leaflet and triforked leaf. The frequency of leaf morphological changes noticed more in SA (0.020%) in Konkan bhushan and EMS (0.15%) in Konkan Wal-2 of Hyacinth bean variety.
Authors and Affiliations
Undirwade GP, Kulkarni GB
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