Effectiveness bacteriolysis of Shiga toxin-producing Escherichia coli O157: H7 caused by specific bacteriophage isolated from pig slurry
Journal Title: Acta Scientiarum Polonorum Zootechnica - Year 2015, Vol 14, Issue 1
Abstract
This study was aimed to in vitro evaluation of a bacteriophage isolated from pig slurry samples as a bacteriolytic agent to elimination Shiga toxin–producing E. coli (STEC) O157: H7. Collected STEC O157: H7 strains was susceptible for bacteriophage infection. The phage infection at the multiplicity of infection (MOI) of 1, 5 or 8 caused a rapid cell lysis resulting in lack of growth in the cell concentration. In conclusion, the data obtained from the present study shows that bacteriophage isolated from pig slurry is a lytic bacteriophage capable to killing strains of E. coli O157: H7. Our results demonstrate that specific bacteriophages have the potential to biocontrol STEC strains in environment.
Authors and Affiliations
Bartłomiej Grygorcewicz, Magdalena Struk, Agata Wasak, Paweł Nawrotek
Keywords
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