Effectiveness of different solutions in decontamination of gutta-percha cones<br /> exposed to Enterococcus faecalis
Journal Title: RSBO - Year 2005, Vol 2, Issue 2
Abstract
The cones of gutta-percha are the main root canal filling materials.The aim of this study was to evaluate the effectiveness of some products in the decontamination of these cones. Eighty (80) cones of gutta-percha #40, separated in 8 groups with 10 cones each one, have been used as sampling. Seventy cones have been contaminated with Enterecoccus faecalis for immersion in saline solution contends 105 to 108 bacterial cells/mL.The groups have suffered decontamination for G1: alcohol 70%; G2: alcohol 70% + iodine 1%; G3: alcohol 70% + chlorexidine 4%; G4: chlorexidine 4%; G5: NaOCl 2,5%; G6: NaOCl 5,25%; G7: saline solution; G8: it was not contaminated and it did not suffer decontamination (control). After 1 minute in contact with each product, five (5) cones have been removed,washed in sterile saline solution and introduced individually in tubes containing BHI solution. The other cones have been removed after 5 minutes in contact with the solutions, repeating the same process of the previous ones. The set of tubes have been agitated per 1 minute and taken to the sterilizer and kept there at 37ºC for 48 hours. The tubes that presented turbidity of BHI solution have been considered positive. It could be observed that in the time of 1 minute there was a bacterial growth in groups 1, 2, 5 and 7 and in the time of 5 minutes only groups 5 and 7 had presented bacterial growth. Group 8 presented one contaminated cone.With the used methodology, we concluded that the alcohol 70% + chlorexidine 4%, chlorexidine 4% and NaOCl 5,25% solutions did not allow the development of E. faecalis, promoting therefore good disinfection of the gutta-percha cones in an adjusted time for clinic practice.
Authors and Affiliations
Flávia FAGUNDES, Denise LEONARDI, Gisele HARAGUSHIKU, Flares BARATTO FILHO, Luiz TOMAZINHO, Paulo TOMAZINHO
Keywords
Related Articles
- EP ID EP160253
- DOI -
- Views 215
- Downloads 0
Pleomorphic adenoma of minor salivary gland: a case report
Introduction: World literature suggests salivary gland tumors account for less than 3% of the head and neck tumors and benign pleomorphic adenoma of minor salivary glands arising de novo is very rare. Objective, ca...
Polyphenols and oral health
Introduction: Polyphenols (PPs) are reactive metabolites abundant in plant derived foods, especially fruits, seeds and leaves. Polyphenols exert preventive activity against infectious and degenerative diseases incl...
In vitro evaluation of the microhardness of normal and sclerotic bovine dentin
Introduction: The literature shows different characteristics between normal and sclerotic dentin. Hardness is a property that has been very used to compare restorative materials and biological tissues. Objective: The aim...
Evaluation of 14 cases of oral squamous cell carcinoma with delayed diagnosis
Introduction: Pathological processes of several causes such as infectious, inflammatory, immunological, allergic, systemic and traumatic diseases often affect the oral cavity. The dentist is responsible for the diagnos...
Ethical and legal implications of marketing in Dentistry
Introduction and objective: The aim of this study was to discuss the ethical and legal use of marketing in dentistry by the professionals. Marketing itself is very important for solving the problems of competitiveness of...