Effectiveness useing of the intracytoplasmic sperm injection for the transmission of genetic information in the japanese quail (сoturnix japonika)
Journal Title: Біоресурси і природокористування [Biological Resources and Nature Management] - Year 2017, Vol 9, Issue 1
Abstract
This study was conducted in order to introduce new methodological approaches transgenesis quail by using intracytoplasmic sperm injection (ICSI). We researched the ability to fertilization and transfection oocytes of quail via microinjection of sperm treated by Triton X-100 with using an extract sperm and CRISPR/Cas-GFP vector.
Authors and Affiliations
Y. Lesniak, L. Kalakaylo, V. Spiridonov, S. Midyk
Keywords
Related Articles
- EP ID EP258089
- DOI -
- Views 133
- Downloads 0
Comparative analysis of genetic structure of ukrainian paddlefish (polyodon spathula) populations
The comparative analysis of the genetic structure of three paddlefish populations was carried out by using microsatellite DNA markers. As the result, Kherson population (25 allelic variants) was the most polymorphic, and...
Sanitary and hygienic conditions for good productivity of organic poultry
The article provides an analytical review of the state of modern organic production in Ukraine, in particular the cultivation of poultry meat production direction. The necessity of conservation of biological diversity of...
METHODS OF DIAGNOSIS AND THE PROVISION OF CONSERVATIVE AND SURGICAL TREATMENT FOR DISCOPATHY IN DOGS
Sick animals with spinal cord injuries make up the heaviest group due to spine and spinal pathologies. In the case of the latter, the spinal cord, the roots of the nerves, as well as the cerebral cords, are damaged, quic...
SPATIOTEMPORAL CHARACTERISTICS OF MICROBIOLOGICAL AND HYDROCHEMICAL INDICATORS OF THE QUALITY OF SURFACE WATERS OF THE UZH RIVER (UKRAINE)
The Uzh River is the main source of water supply for the regional center - the city of Uzhhorod and the surrounding villages. Over the past decades, there has been a deterioration in the ecological status of the reservoi...
Growth of calves in the milking period with an increase in the content of lysine and methionine in the milk replacer
The relevance of the study is due to the need to optimize the content of lysine and methionine in milk replacer (MR) for calves. The aim of the work is to study the effect of additives of synthetic L-lysine and DL-methio...