EFFECTS OF ANESTHESIA ON INNATE IMMUNE COMPONENTS IN ORTHOPEDIC SURGERY
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2018, Vol 11, Issue 12
Abstract
Objectives: The present study was carried out to evaluate the possible role of anesthetics and surgery on innate by measuring the levels of tumor necrosis factor alpha (TNFα) and interferon gamma (IFNγ) by enzyme-linked immunosorbent assay (ELISA). Flow cytometry was used to determine MCP-I and CD16.Methods: Serum level of TNFα and IFNγ cytokines was measured using ELISA technique. Flow cytometry was used to determine MCP-I and CD16. White blood cell count and C-reactive protein (CRP) were determined.Results: There was a highly significant rise in CRP blood level postoperatively when compared with pre-operative blood level (p<0.001). In addition, the results revealed a significant rise in neutrophil count after operation in comparison with its baseline level before operation (p<0.001), while the level of lymphocytes showed significant decline following operation (p<0.001). The level of monocyte and eosinophil also got significantly reduced after operation (p<0.001), whereas the level of basophil showed no significant alteration after operation (p=0.687). CD16 NK cell count revealed a significant rise during the time of anesthesia. However, it showed a significant decrease postoperatively, but the reduction did not reach baseline count. It has been noticed also that the level of MCP-1 got raised significantly in association with anesthesia induction; however, it returned back to its baseline level following surgery.Conclusions: Analysis of data to correlate the innate immune components TNFα, IFNγ, MCP-I, and CD16 with types of anesthetic drugs (general, local, and regional anesthesia) showed no significant association between these components and type of anesthesia (p>0.05).
Authors and Affiliations
Adnan Hamad Aubaid, Khalid Lahmood Yassen
Keywords
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- EP ID EP605263
- DOI 10.22159/ajpcr.2018.v11i12.29554
- Views 137
- Downloads 0
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