Effects of Astragalus polysaccharide on immune function in B16 melanoma mice
Journal Title: TMR Integrative Medicine - Year 2021, Vol 5, Issue 12
Abstract
Objective: To examine the effects of Astragalus polysaccharide on immune function in B16 melanoma mice. Method: Forty male C57BL/6 mice were divided equally into a control group, model group, Astragalus polysaccharide low-dose group, and Astragalus polysaccharide high-dose group, with 10 mice per group. B16 cells were used to develop a mouse model of melanoma. After B16 engraftment, 40 mg/kg and 80 mg/kg Astragalus polysaccharide was administered by gavage every day to the Astragalus polysaccharide low-dose group and Astragalus polysaccharide high-dose group, respectively. Splenic index, thymic index, tumor growth curves, and tumor inhibition rates were measured. Flow cytometry was used to measure proportions of peripheral blood T lymphocyte subsets. Hematoxylin and eosin staining was used to examine histopathological changes in tumors. Immunofluorescence double staining was used to identify myeloid-derived suppressor cells in tumor tissues. Results: In the Astragalus polysaccharide high-dose group, splenic and thymic indices were significantly increased and tumor growth was inhibited in melanoma mice. Flow cytometry demonstrated increased CD4+ and CD4+/CD8+T-cell ratios in the high-dose Astragalus polysaccharide group. HE staining demonstrated significantly decreased numbers of tumor cells among mice with melanoma in the high-dose Astragalus polysaccharide group. Immunofluorescence double staining demonstrated significantly decreased numbers of myeloid-derived suppressor cells in tumor tissues in the high-dose Astragalus polysaccharide group. Conclusion: Astragalus polysaccharide inhibits tumor growth, increases splenic and thymic indices, increases CD4+ and CD4+/CD8+ T-cell ratios, and decreases myeloid-derived suppressor cell numbers in melanoma mice. Our results indicate Astragalus polysaccharide may enhance immune function resulting in inhibition of tumorigenesis and tumor progression.
Authors and Affiliations
Fang-Hua Wu, Kui Chen, De-Kun Zhang, Chao Gong, Jiang-Yue Yu, Shen-Zhou Huang, Chen-Xin Huang, Li-Qun Wang
Keywords
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- EP ID EP696227
- DOI 10.12032/TMRIM202105012
- Views 88
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