Effects of different medium on morphophysiological traits of Lippia citriodora H.B.K. during in vitro culture condition
Journal Title: Journal of Herbal Drugs - Year 2014, Vol 5, Issue 3
Abstract
Background & Aim: Lemon verbena (Lippia citriodora H.B.K.) is a richly scented herb and has a long history as a sacred and medicinal plant. Select an appropriate medium in tissue culture is a necessity for success Experimental: To evaluate the effect of 10 medium of previous researches for Lippia micropropagation (1- MS medium supplemented with BAP (3 mgL-1) + IBA (0.1 mgL-1) + activated charcoal (1 gL-1) 2- MS medium supplemented with BAP (1 mgL-1) + IBA (0.5 mgL-1) + activated charcoal (1 gL-1) 3- 1/2MS 4- MS medium supplemented with BAP (5 mgL-1) 5- MS medium supplemented with BAP (1 mgL-1) 6- MS medium supplemented with activated charcoal (1 gL-1) 7- MS medium supplemented with BAP (1 mgL-1) + activated charcoal (1 gL-1) 8- MS medium supplemented with BAP (1 mgL-1) + AgNO3 (5 mgL-1) 9- MS medium supplemented with AgNO3 (5 mgL-1) 10- MS (control)), an experiment was conducted as a Completely Randomized Design (CRD) with three replicates and three explants per replication. Results & Discussion: The results showed positive effects for BAP (5 mgL-1) and somewhat active charcoal, but contrary to predictions, AgNO3 had no effect on reducing senescence and leaf necrosis. Recommended applications/industries: Therefore, Based on the finding, the four medium (MS medium supplemented with BAP (5 mgL-1)) with the highest rates of shoot fresh weight, leaf number, internodes number, shoot number, root fresh weight, callus diameter and root dry weight can be introduced as a suitable medium of tissue culture in this experimental conditions.
Authors and Affiliations
Hassan Nourafcan; Fatemeh Sefidkon; Amir Mousavi; Mozaffar Sharifi; Ahmad Khalighi
Keywords
Related Articles
- EP ID EP23455
- DOI -
- Views 347
- Downloads 18
Bioactivities of essential oil of Eucalyptus globolus L. against Tribolium castaneum
Background & Aim: The use of herbal extracts as safe and effective factors for eradicating of stores pests has attracted much attention. In this paper, the bio-effects of fumigation and repellent effects of essential oil...
Antioxidant activity of essential oil of Heracleum lasiopetalum fruits on chemical properties of potato chips
Background & Aim: Chemicals antioxidants and lipid oxidation products are known as cancer-causing factors in food systems. In this study, the effect of essential oil from Heracleum lasiopetalum fruits on lipid oxidation...
Study the ethnobotany of medicinal plants in Sirjan, Kerman province, Iran
Background & Aim: Since traditional knowledge is base of producing new herbals and other natural products, and old people of ancient cities know about characteristics of such herbals, therefore, death of such people will...
Essential oil composition of Tanacetum chiliophyllum var. oligocephalum from Iran
Background & Aim: The genus Tanacetum L. belongs to the family Asteraceae and tribe Anthemideae. According to the recent findings the number of Tanacetum species in Iran is 36, of which 12 are endemic. In this study the...
Effect of the extract of Hypericum perforatum on digestive organs morphometry in Wistar rats
Background & Aim: St John's wort (Hypericum perforatum L.) contains hypericin and hyperforin as the main components of the extract that binds with GABA and sigma opioid receptors. This herb has many pharmacological effec...