Effects of different storage conditions on the content of key growth factors in platelet-rich plasma
Journal Title: Chinese Journal of Blood Transfusion - Year 2023, Vol 36, Issue 8
Abstract
Objective To study the effects of different storage temperature and different storage time on the activity of key growth factors in platelet-rich plasma(PRP), and to provide a theoretical basis for maximize the role of PRP in clinical treatment. Methods PRP was collected by blood cell isolation and apheresis, stored at 22℃ and -80℃, respectively. VEGF, TGF-β and PDGF were detected by ELISA. The content of growth factors in PRP was detected when stored at 22℃for 1, 3 and 5 days, and the growth factors content of PRP stored at 22℃ for 3 days was detected after thrombin activation for 0.5, 1 and 1.5 hours. The content of growth factor in frozen PRP (stored at -80℃ for 30 days after initial 3-days storage at 22℃ ) and fresh PRP (stored at 22℃ for 3 days) was compared. The growth factor content in PRP frozen at - 80℃ for 30, 60 and 180 days, and the growth factor content in PRP frozen at -80℃ for 180 days after repeated freeze-thaw for 1, 2, 3, 5 and 10 times were detected. Results The growth factor content of apheresis PRP was significantly higher than that of platelet-poor plasma. No statistical difference was noticed in VEGF, TGF-β and PDGF content in PRP at 1, 3 and 5 days stored at 22℃; no statistical difference was noticed in VEGF, TGF-β and PDGF content in PRP stored at 22℃ for 3 days after thrombin activation for 0.5, 1 and 1.5 hours. There was no statistically significant difference in growth factor content between PRP stored at 22℃ for 3 days versus frozen at -80℃ for 30 days after initial 3-days storage at 22℃. No statistical difference was found in VEGF, TGF-β and PDGF contents in frozen PRP repeatedly frozen and thawed for 1 to 10 times. Conclusion Apheresis PRP can release a large amount of growth factors after activation. Fresh PRP stored at 22℃ for 5 days or frozen at -80℃ for 180 days has no impact on the content of growth factors, and frozen PRP at -80℃ can achieve long-term, effective and safe preservation, which is conducive to multiple use of PRP in treatment.
Authors and Affiliations
Wen WU, Hongxia MA, Xiangyan HUANG
Keywords
Related Articles
- EP ID EP738604
- DOI 10.13303/j.cjbt.issn.1004-549x.2023.08.001
- Views 44
- Downloads 0
Application of machine learning in predictive analysis of blood usage for liver transplantation surgery
Objective To explore the application of machine learning in scientific and rational blood preparation and predictive analysis for surgical blood usage before liver transplantation surgery. Methods Clinical basic informat...
Automatic transfer of diversion pouches among Blood Transfusion Departments: a multicenter observation and study
Objective To explore the significance of automatic transfer of diversion pouches in blood transfusion departments on improving the standardization of quality management. Methods A total of 16 548 diversion pouches of sus...
Application of ARIMA model in predicting pediatric red blood cell usage
Objective To explore the feasibility of using autoregressive moving average model (ARIMA) to predict the dosage of suspended red blood cells in children, and to provide a basis for the development of clinical blood reser...
Quality analysis of fresh frozen plasma prepared by the improved ultra-low temperature storage box
Objective To observe and analyze the influence of the improved ultra-low temperature storage box on the quality of fresh frozen plasma (FFP). Methods A total of 80 qualified whole blood samples (400 mL, O type not includ...
Investigation of human parvovirus B19 infection in college students
Objective To investigate the prevalence of human parvovirus B19 (B19V) infection among college students, so as to provide reference for the development of blood donor screening strategies and blood supply policies. Metho...