Effects of stress pretreatment on the dynamics of blood cholinesterase activity after exposure to an organophosphorus pesticide in the rat.
Journal Title: Annals of Agricultural and Environmental Medicine - Year 2010, Vol 17, Issue 1
Abstract
A single i.p. administration of 1.0 mg/kg of chlorphenvinphos (CVP), an organophosphorus pesticide, results in an acute stress response, evidenced by a marked (6-7 fold) rise in plasma corticosterone (CORT) concentration, and a diminished behavioural sensitivity to amphetamine (AMPH) three weeks postexposure. Surprisingly, in rats subjected to a single series of inescapable electric footshocks (60 10 msec triplets of 3.0 mA, 2 msec, square pulses during 20 min - IF ) two weeks prior to the CVP exposure, these effects are not observed. It has been assumed that the reduced effectiveness of CVP might be related to some persisting alterations in the functional state of the cholinergic system. The aim of the present work was to discover whether and in what way the IF pretreatment affects i) the cholinesterase activity in blood, and ii) the dynamics of the alterations in the cholinesterase (ChE) activity following the CVP exposure. The experiments were performed on 3 mo. old, male Wistar rats. In the first experiment, the blood samples were taken from the tail vein 15, 60 and 180 min after the IF. In the second experiment, the rats were pretreated with IF and 14 days later given 1.0 mg/kg of CVP i.p. Blood samples were taken 15 min, 60 min, 180 min, 24 h, 7 days, and 14 days after the CVP exposure. In the first experiment no differences in the ChE activity in plasma (pChE) and erythrocytes (rbcChE) were found between the shocked and control rats. In the second experiment, however, in rats pretreated with IF the rbcChE activity of was reduced by CVP less and pChE activity returned to normal faster than in rats not pretreated with IF. The results confirm that exposure to IF, a nonchemical stressor, induces some long-lasting adaptive changes which render the cholinergic system less susceptible to the harmful action of ChE inhibitors. It has been hypothesized that the changes consist in an increase of the antioxidant potential in blood and possibly other tissues.
Authors and Affiliations
Slawomir Gralewicz, Radoslaw Swiercz, Piotr Lutz, Dorota Wiaderna, Wojciech Wasowicz
Keywords
Related Articles
- EP ID EP58671
- DOI -
- Views 136
- Downloads 0
Serological survey in persons occupationally exposed to tick-borne pathogens in cases of co-infections with Borrelia burgdorferi, Anaplasma phagocytophilum, Bartonella spp. and Babesia microti
Sera of 39 farmers, 119 foresters and 32 blood donors were investigated for the presence of antibodies against [i]B. burgdorferi[/i], [i]A. phagocytophilum[/i], [i]B. microti[/i] and [i]Bartonella[/i] spp. Semi-quantitat...
Association between PM10 air pollution and birth weight after full-term pregnancy in Krakow city 1995–2009 – trimester specificity
Introduction and objective. The results of epidemiological studies indicate that the higher maternal exposure to air pollution, especially with particulate matter during pregnancy, the lower the infant’s birth weight. Th...
Effect of repetitive mowing on common ragweed (Ambrosia artemisiifolia L.) pollen and seed production.
Ambrosia artemisiifolia L (common ragweed) is a familiar roadside weed in southern Quebec (Canada) that produces large amounts of airborne pollen responsible for multiple rhino-conjunctivitis (hay fever) cases. As roadsi...
Environmental conditioning of incidence of tick-borne encephalitis in the south-eastern Poland in 1996-2006.
Epidemiological studies of tick-borne encephalitis (TBE) conducted in the Swietokrzyskie province (south- eastern Poland) in 1996-2006 demonstrated an increase in the number of registered TBE cases in the last 6 years of...
Determinants of sense of coherence among managerial nursing Staff
[b]Introduction.[/b] The sense of coherence is a tool for confronting the challenges posed by life. It constitutes a general human orientation, which is expressed through the conviction that outside stimuli can be explai...