Effects of the human CD38 glycoprotein on the early stages of the HIV-1 replicationcycle.
Journal Title: The FASEB Journal - Year 1999, Vol 13, Issue 15
Abstract
CD38 displays lateral association with the HIV-1 receptor CD4. This associationis potentiated by the HIV-1 envelope glycoprotein gp120. The aim of this work was to evaluate the CD38role in T cell susceptibility to HIV-1 infection. Using laboratory X4 HIV-1 strains and X4 and X4/R5primary isolates, we found that CD38 expression was negatively correlated to cell susceptibility to infection,evaluated as percentage of infected cells, release of HIV p24 in the supernatants, and cytopathogenicity.This correlation was at first suggested by results obtained in a panel of human CD4(+) T cell lines expressingdifferent CD38 levels (MT-4, MT-2, C8166, CEMx174, Supt-1, and H9) and then demonstrated using CD38 transfectantsof MT-4 cells (the line with the lowest CD38 expression). To address whether CD38 affected viral binding,we used mouse T cells that are non-permissive for productive infection. Gene transfection in mouse SR.D10.CD4(-).F1T cells produced four lines expressing human CD4 and/or CD38. Ability of CD4(+)CD38(+)cells to bind HIV-1or purified recombinant gp120 was significantly lower than that of CD4(+)CD38(-) cells. These data suggestthat CD38 expression inhibits lymphocyte susceptibility to HIV infection, probably by inhibiting gp120/CD4-dependentviral binding to target cells.-Savarino, A., Bottarel, F., Calosso, L., Feito, M. J., Bensi, T., Bragardo,M., Rojo, J. M., Pugliese, A., Abbate, I., Capobianchi, M. R., Dianzani, F., Malavasi, F., and Dianzani,U. Effects of the human CD38 glycoprotein on the early stages of theHIV-1 replication cycle.
Authors and Affiliations
A Savarino, F Bottarel, L Calosso, M J Feito, T Bensi, M Bragardo, J M Rojo, I Abbate, M R Capobianchi, U Dianzani
Keywords
Related Articles
- EP ID EP83578
- DOI -
- Views 132
- Downloads 0
Serotoninergic and melatoninergic systems are fully expressed in human skin.
We investigated the cutaneous expression of genes and enzymes responsible for the multistep conversion of tryptophan to serotonin and further to melatonin. Samples tested were human skin, normal and pathologic (basal cel...
Effects of the human CD38 glycoprotein on the early stages of the HIV-1 replicationcycle.
CD38 displays lateral association with the HIV-1 receptor CD4. This associationis potentiated by the HIV-1 envelope glycoprotein gp120. The aim of this work was to evaluate the CD38role in T cell susceptibility to HIV-1...